摘要
Fibroblast growth factor 21(FGF21)serves as an essential biomarker for early detection and diagnosis of nonalcoholic fatty liver disease(NAFLD).It has received a great deal of attention recently in efforts to develop an accurate and effective method for detecting low levels of FGF21 in complex biological settings.Herein,we demonstrate a label-free,simple and high-sensitive field-effect transistor(FET)biosensor for FGF21 detection in a nonaqueous environment,directly utilizing two-dimensional molybdenum disulfide(MoS2)without additional absorption layers.By immobilizing anti-FGF21 on MoS2 surface,this biosensor can achieve the detection of trace FGF21 at less than 10 fg mL-1.High consistency and satisfactory reproducibility were demonstrated through multiple sets of parallel experiments for the MoS2 FETs.Furthermore,the biosensor has great sensitivity to detect the target FGF21 in complex serum samples,which demonstrates its great potential application in disease diagnosis of NAFLD.Overall,this study shows that thin-layered transition-metal dichalcogenides(TMDs)can be used as a potential alternative platform for developing novel electrical biosensors with high sensitivity and selectivity.
成纤维细胞生长因子21(FGF21)是一种用于早期检测和诊断非酒精性脂肪肝病(NAFLD)的必需生物标志物.最近,开发在复杂生物环境中准确有效地检测血液中低浓度FGF21的方法受到了极大的关注.本文展示了一种无标记、简单和高灵敏度的场效应晶体管(FET)生物传感器,用于在非水环境中检测FGF21.通过对二硫化钼(MoS2)表面进行功能化将抗FGF21牢固地固定在MoS2材料上,使该生物传感器实现检测FGF21的检测限小于10 fg mL^-1.多组平行实验证明了MoS2 FET的高度一致性和令人满意的再现性.此外,生物传感器对复杂血清样品中的目标FGF21具有很高的敏感度,这表明其在NAFLD疾病诊断中具有巨大的潜在应用前景.
作者
Xinxing Gong
Yeru Liu
Haiyan Xiang
Hang Liu
Zhigang Liu
Xiaorui Zhao
Jishan Li
Huimin Li
Guo Hong
Travis Shihao Hu
Hong Chen
Song Liu
Gang Yu
龚新星;刘业茹;向海燕;刘航;刘志刚;赵晓蕊;李继山;李惠敏;洪果;Travis Shihao Hu;陈洪;刘松;余刚(Institute of Chemical Biology and Nanomedicine(ICBN),State Key Laboratory of Chemo/Biosensing and Chemometrics,College of Chemistry and Chemical Engineering,Hunan University,Changsha 410082,China;Department of Head and Neck Oncology,The Cancer Center of the Fifth Affiliated Hospital of Sun Yat-sen University,Phase I Clinical Trial Laboratory,The Fifth Affiliated Hospital,Sun Yat-sen University,Zhuhai 519001,China;Institute of Applied Physics and Materials Engineering,University of Macao,Avenida da Universidade,Taipa,Macao,China;Department of Mechanical Engineering,California State University,Los Angeles,CA 90032,USA;School of Materials Science and Energy Engineering,Foshan University,Foshan 528000,China)
基金
financially supported by the National Natural Science Foundation of China(21705036,21475036,51271074,21476066,and 81572500)
the Natural Science Foundation of Hunan Province,China(2018JJ3035)
Hunan Young Talents(2016RS3036)
the Fundamental Research Funds for the Central Universities from Hunan University
the Start-up Research Grant(SRG2016-00092-IAPME),Multi-year Research Grant(MYRG2018-00079-IAPME)of the University of Macao
Science and Technology Development Fund(081/2017/A2,0059/2018/A2,009/2017/AMJ),Macao SAR(FDCT)
