摘要
目的探讨幽门螺杆菌(H.pylori)感染与自身免疫性肝病(AILD)的相关性。方法选取2016年7月至2018年7月我院首次诊断AILD患者60例。入院后治疗前采用13 C尿素呼气试验(^13 C-UBT)检测患者H.pylori感染情况,酶联免疫吸附试验(ELISA)法检测患者血清抗髓过氧化物酶抗体(MPO)含量,间接免疫荧光法(IIF)检测患者抗核抗体(ANA)、抗线粒体抗体(AMA)、抗平滑肌抗体(SMA)和抗中性粒细胞胞浆抗体(ANCA)阳性率,免疫印迹法(IBT)检测患者抗线粒体Ⅱ型抗体(AMA-M2)、抗肝肾微粒体抗体Ⅰ型(LKM-1)、抗肝胞浆I型抗体(LC-1)和抗可溶性肝抗原/肝胰抗原(SLA/LP)阳性率;全自动生化分析仪检测患者肝功能指标:谷丙转氨酶(ALT)、谷草转氨酶(AST)、碱性磷酸酶(ALP)和谷氨酰转移酶(GGT)含量;ELISA法检测患者血清细胞因子IFN-γ、IL-6、IL-10和TNF-α含量。结果共检出H.pylori阳性37例(61.67%)。H.pylori阳性患者MPO阳性率明显高于H.pylori阴性患者(P<0.05);H.pylori阳性患者ANA、AMA、SMA、ANCA阳性率明显高于H.pylori阴性患者(P<0.05);H.pylori阳性患者AMA-M2、LKM-1、LC-1、SLA/LP阳性率明显高于H.pylori阴性患者(P<0.05);H.pylori阳性患者ALT、AST、ALP、GGT水平与H.pylori阴性患者比较差异无统计学意义(P>0.05);H.pylori阳性患者IFN-γ、IL-6、IL-10/TNF-α水平明显高于H.pylori阴性患者(P<0.05)。结论AILD患者H.pylori感染率较高,且与多种免疫性抗体阳性和细胞因子水平升高密切相关。
Objective To study the correlation between Helicobacter pylori(H.pylori)infection and autoimmune liver disease(AILD).Methods A total of 60 consecutives firstly diagnosed as AILD patients were enrolled,then to detect H.pylori infection with ^13 C urea breath test(^13 C-UBT),serum anti myeloperoxidase antibody(MPO)level with Enzyme linked immunosorbent assay(ELISA),positive rates of anti-nuclear antibody(ANA),anti-mitochondrial antibody(AMA),anti-smooth muscle antibody(SMA)and anti-neutrophil cytoplasmic antibody(ANCA)with Indirect immunofluorescence(IIF),Positive rates of anti-mitochondrial antibody type II(AMA-M2),anti-hepatorenal microsomal antibody type I(LKM-1),anti-hepatocytoplasmic antibody type I(LC-1)and anti-soluble hepatic antigen/hepatopancreatic antigen(SLA/LP)with immune blotting test(IBT),Liver function indicators including Glutamic pyruvic transaminase(ALT),Glutamic pyruvic transaminase(AST),Alkaline phosphatase(ALP)and Glutaminyl transferase(GGT)levels with Automatic biochemical analyzer,Serum cytokines IFN-γ,IL-6,IL-10 and TNF-αlevels with ELISA.Results There were up to 37 cases(61.67%)of H.pylori positive,the MPO positive rate in H.pylori positive group was significantly higher than H.pylori negative group,positive rates of ANA,AMA,SMA,ANCA,AMA-M2,LKM-1,LC-1 and SLA/LP were all more,too(P<0.05).Though the levels of ALT,AST,ALP and GGT in the two groups were no statistical differences(P>0.05),the levels of IFN-γ,IL-6,IL-10 and TNF-αin HP positive group were higher too(P<0.05).Conclusion The more HP infection positive in AILD patients,which is related to higher multiple positive immune antibodies and cytokines.
作者
张冉冉
朱传会
ZHANG Ran-ran;ZHU Chuan-hui(Department of gastroenterology,Mingji Hospital Affiliated to Nanjing Medical University,Nanjing 210019,Jiangsu,China)
出处
《现代消化及介入诊疗》
2019年第8期842-846,共5页
Modern Interventional Diagnosis and Treatment in Gastroenterology
基金
江苏省南京市医学科技发展项目(YKK17251)
关键词
幽门螺杆菌
自身免疫性肝病
肝功能
细胞因子
Helicobacter pylori
Autoimmune liver disease
Liver function
Cytokines
