摘要
目的建立珐菲亚中齐墩果酸含量测定的方法,并比较珐菲亚中不同生长年限和不同部位齐墩果酸含量的异同。方法采用高效液相色谱法,色谱柱为Phenomenex Luna C1(8 250 mm×4.6 mm,5μm);流动相为甲醇-水-冰乙酸-三乙胺(285∶15∶0.2∶0.1,V/V);检测波长为210 nm,柱温为30℃,流速为1.0 ml/min,进样量为20μl。采用建立的方法测定珐菲亚中齐墩果酸的含量,并采用正交实验优化齐墩果酸的提取工艺。结果齐墩果酸质量浓度线性范围为0.0206~2.060 mg/ml(r^2=1.0000),精密度、重复性、稳定性实验的RSD均<2%(n=6或n=7),齐墩果酸平均回收率为100.89%(RSD=1.69%,n=9)。最佳提取条件为:以20倍量80%乙醇回流提取1.5 h,提取3次,3.0%硫酸(g/g)酸水解1 h时,齐墩果酸提取效率最高。齐墩果酸在珐菲亚的芦头、根、老茎、嫩茎、叶和花中的含量分别为:4.87、4.61、2.67、0.99、0.24和1.13 mg/g。1、2、3、4和5年生的广西产珐菲亚根部中,齐墩果酸的平均含量分别为3.08、4.07、4.71、4.62和4.46 mg/g。结论所建立的检测方法和提取工艺适用于珐菲亚中齐墩果酸的提取与含量检测。齐墩果酸在珐菲亚不同部位中均有分布,但含量差异明显,芦头中含量最高,叶中含量最低。3年生长期后的广西产珐菲亚中齐墩果酸含量基本达到稳定。
Objective To establish a method for the determination of oleanolic acid(OA)in Pfaffia glomerata to compare the contents of OA in different parts of P.glomerata from Guangxi,China,with different growth years and dif?ferent habitats.Methods HPLC method was adopted.The determination was performed on Phenomenex Luna C18(250 mm×4.6 mm,5μm)column with mobile phase consisting of methanol-water-glacial acetic acid-triethylamine(285∶15∶0.2∶0.1,V/V)at the flow rate of 1.0 ml/min.The column temperature was set at 30℃.The detection wavelength was set at 210 nm,and the injection volume was 20μl.The content of OA in P.glomerata was determined by the established method,and the extraction process of OA was optimized by the orthogonal test.Results The linear range of OA was 0.0206-2.060 mg/ml(r^2=1.0000).RSDs for the precision,repeatability and stability tests were all lower than 2%(n=6 or n=7).The average recovery of OA was 100.89%(RSD=1.69%,n=9).The optimum extraction conditions of OA were as follows:20-fold ethanol(80%,V/V),extracting for three times,refluxing 1.5 hour each time,and processing acid hydrolysis with 3.0%sulfuric acid(g/g)for 1 hour.Under these conditions,the OA had the highest extraction efficiency.The contents of OA in reed head,root,old stem,tender stem,leaf and flower of P.glomerata were 4.87,4.61,2.67,0.99,0.24 and 1.13 mg/g,respectively.The average contents of OA in the roots of P.glomerata aged 1,2,3,4 and 5 years in Guangxi were 3.08,4.07,4.71,4.62 and 4.46 mg/g,respectively.Conclusion The established extraction process and detection method is suitable for the extraction and content determination of OA in P.glomerata.Although OA is distributed in all parts of P.glomerata,the contents significantly vary in different parts.The content of OA is highest in reed head and lowest in leaves.The OA content in P.glomerata becomes stable after 3 years of growth in Guangxi.
作者
许崇摇
黄程
潘波
朱丹
王德宝
韦霞
韦贵云
XU Chong-yao;HUANG Cheng;PAN Bo;ZHU Dan;WANG De-bao;WEI Xia;WEI Gui-yun(School of Pharmacy,Guangxi Medical University,Nanning 530021,China;Guangxi Zhuang Autonomous Region Forestry Science Research Institute,Nanning 530002,China;Guangxi Zhuang Autonomous Region Maternal and Child Health Hospital,Nanning 530003,China)
出处
《国际药学研究杂志》
CAS
北大核心
2019年第5期393-397,共5页
Journal of International Pharmaceutical Research
基金
广西医科大学青年科学基金资助项目(GXMUYSF201605)
广西科技基地和人才专项资助项目(0863-2)
广西大学生创新创业训练计划项目(201510598043,201610598183,201710598150)
