摘要
目的探讨微小RNA(miR)?122和miR?150在隐匿性乙型肝炎鉴别诊断中的应用效果。方法选择本院2018年3月至2019年2月收治的45例隐匿性乙型肝炎患者(A组)、60例慢性乙型肝炎患者(B组)和50例健康人(C组)作为研究对象,检测所有受检者清晨空腹静血血清miR?122和miR?150水平。以血清miR?122和miR150的Ct值作为自变量,以是否为隐匿性乙型肝炎作为因变量,采用受试者工作特征(ROC)曲线分析miR?122和miR?150水平鉴别诊断隐匿性乙型肝炎感染的效能,绘制ROC曲线,确定最佳截断值并计算敏感度、特异度、约登指数、阳性预测值、阴性预测值和符合率。结果A组和B组血清miR?122和miR?150的Ct值显著低于C组,差异有统计学意义(P<0.05);而A组和B组比较,二者差异无统计学意义(P>0.05)。血清miR?122的AUC为0.949(95%CI:0.903~0.991),最佳截断值为Ct=28,敏感度、特异度、约登指数、阳性预测值、阴性预测值和符合率分别为95.56%、88.00%、83.56%、83.67%、95.65%和91.58%。血清miR?150的AUC为0.937(95%CI:0.893~0.985),最佳截断值为Ct=26,敏感度、特异度、约登指数、阳性预测值、阴性预测值和符合率分别为91.11%、90.00%、81.11%、89.13%、91.84%和90.53%。结论血清miR?122和miR?150鉴别诊断隐匿性乙型肝炎具有良好的敏感性和特异性。
Objective To investigate the application of microRNA(microRNA)122 and miR?150 in the differential diagnosis of occult hepatitis B.Methods 45 patients with occult hepatitis B(group A),60 patients with chronic hepatitis B(Group B)and 50 healthy persons(Group C)were selected as the study subjects from March 2018 to February 2019.The levels of serum miR?122 and miR?150 were detected in all the subjects.Receiver Operating Characteristic(ROC)curve was used to analyze the efficiency of differentiating the levels of miR?122 and miR?150 in the diagnosis of latent hepatitis B infection.ROC curve was drawn to determine the best cut?off value and calculate sensitivity,specificity,Yoden index,positive predictive value,negative predictive value and coincidence rate.Results The Ct values of serum miR?122 and miR?150 in group A and group B were significantly lower than those in group C(P<0.05).There was no significant difference between the A group and the B group(P>0.05).The AUC of serum miR?122 was 0.949(95%CI:0.903~0.991),the optimal cutoff value was Ct=28,sensitivity,specificity,Yoden index,positive predictive value,negative predictive value and coincidence rate were 95.56 respectively.%,88.00%,83.56%,83.67%,95.65%and 91.58%.The AUC of serum miR?150 was 0.937(95%CI:0.893~0.985),the optimal cutoff value was Ct=26,and the sensitivity,specificity,Yoden index,positive predictive value,negative predictive value and coincidence rate were 91.11%,90.00%,81.11%,89.13%,91.84%and 90.53%,respectively.Conclusion The differential diagnosis of occult hepatitis B by serum miR?122 and miR?150 has good sensitivity and specificity.
作者
马青松
张云芬
MA Qingsong;ZHANG Yunfen(Clinical Laboratory,Qian'an Hospital of TCM,Qian'an,Hebei,China,064400;Metabolism Department,Qian'an Hospital of TCM,Qian'an,Hebei,China,064400)
出处
《分子诊断与治疗杂志》
2019年第5期418-421,427,共5页
Journal of Molecular Diagnostics and Therapy