摘要
碘(I-3)对罗丹明B(RB)具有荧光猝灭作用,可使RB的荧光信号强度减弱甚至消失,而青霉素碱性水解产物青霉噻唑酸可将I-3还原为I-,使体系荧光信号再现,据此建立了以RB-I-3缔合物为荧光探针测定药剂中青霉素含量的新方法。在激发波长365 nm,发射波长580 nm条件下进行了青霉素的荧光测定。结果表明,在0~6μmol/L范围内,青霉素浓度与荧光强度变化值呈良好的线性关系,检出限为1.88×10^-9 mol/L。方法的加标回收率为97.0%~106%,相对标准偏差(n=6)为2.6%~3.1%。该方法简便快速、准确可靠、灵敏度高,可用于药剂中青霉素的含量分析。
A fluorescent probe for the detection of penicillin in medicaments was fabricated with iodine-rhodamine B(RB)associate,as the fluorescence signal intensity of RB would be weakened by iodine(I-3)solution or even disappear,while penicillin alkaline hydrolysis product penicillium thiazole could reduce I-3to I-,making the system fluorescent signal reappear.The fluorescent probe was applied in the determination of penicillin in medicaments at an excitation wavelength of 365 nm and an emission wavelength of 580 nm.Results showed that there was a good linear relationship between fluorescence intensity change and penicillin concentration in the range of 0-6μmol/L with a limit of detection(LOD)of 1.88×10-9 mol/L.The recoveries were in the range of 97.0%-106%with relative standard deviations(n=6)of 2.6%-3.1%.The proposed method was simple,rapid,accurate and reliable,and could be used for the analysis of penicillin in medicaments.
作者
张爱菊
戴兴德
张小林
ZHANG Ai-ju;DAI Xing-de;ZHANG Xiao-lin(Public Course Teaching Department,Gansu Medical College,Pingliang 744000,China)
出处
《分析测试学报》
CAS
CSCD
北大核心
2019年第9期1132-1135,共4页
Journal of Instrumental Analysis
基金
甘肃省高等学校科研项目(2018A-145)
