摘要
目的研究转GmDREB3抗旱基因小麦对大鼠生殖发育的影响。方法无特定病原体(SPF级)Wistar大鼠随机分为基础饲料组、亲本对照组、转基因组,每组60只,雌雄各半,分别给予基础饲料、亲本对照饲料和转基因饲料,13周后组内雌雄大鼠交配产生F1代仔鼠,分析指标有:F0代和F1代大鼠的毒性表现;F0代大鼠的体质量、进食量、食物利用率;F0代雌鼠繁殖指数和性激素水平(促黄体生成素释放激素、卵泡刺激素、雌二醇);F0代雌雄大鼠脏器系数、肝脑比、脾脑比及生殖器官病理;F1代仔鼠体质量、身长、尾长、出生后21天神经病理(大脑、小脑和脑干)和出生后56天雌鼠免疫学指标(分泌性免疫球蛋白A、免疫球蛋白E、溶菌酶、二胺氧化酶)。结果与亲本对照组比较,F0代雄鼠肝脏系数降低(P<0.01)、脾脏系数升高(P<0.05)、脾脑比升高(P<0.01),均差异有统计学意义;F1代仔鼠出生后第0天、第4天、第7天、第21天的尾长偏短,差异有统计学意义(P<0.05),其他指标均差异无统计学意义(P>0.05)。结论转GmDREB3抗旱基因小麦对大鼠生殖发育无毒性作用。
Objective To evaluate reproduction toxicity of GmDREB3 gene modified wheat in Wistar rats.Methods One hundred and eighty specific pathogen free healthy Wistar rats were randomly divided into basic food control group,non-Gm wheat group and Gm wheat group,with 30 male and 30 female in each group,fed with AIN 93 diet,non-Gm wheat and Gm wheat,respectively.After 13 weeks,the rats mated with the same group and produced F1 baby rats.The following indexes were analyzed:the toxic manifestations of the F0 and F1,body weight,food utilization ratio,female hormone level,pathological changes of reproductive organ,viscera coefficient,the ratio of liver and brain,the ratio of spleen and brain,the reproduction indexes of F0.Body weight,body length,tail length,pathological changes of nerve on post natal day 21(PND21)and immunological index on post natal day 56(PND56),including lysozyme(LYS),diamine oxidase(DAO),secretory immunoglobulin A(sIgA)and immunoglobulin E(IgE),were examined in F1 female rats.Results Compared with non-Gm groups,the liver coefficient decreased(P<0.01),spleen coefficient increased(P<0.05)and the ratio of spleen and brain increased(P<0.01)in F0 male rats,tail length of F1 rats in Gm wheat group were decreased(P<0.05)on PND0,PND4,PND7 and PND21.The other indexes in Gm wheat group rats had no obviously difference.ConclusionGmDREB3 gene modified wheat has no reproductive development toxicity in rats.
作者
田洁
柯翔鸿
田梦醒
袁媛
杨文祥
唐晓荞
卓勤
杨晓光
刘家发
樊柏林
TIAN Jie;KE Xianghong;TIAN Mengxing;YUAN Yuan;YANG Wenxiang;TANG Xiaoq iao;ZHUO Qin;YANG Xiaoguang;LIU Jiafa;FAN Bolin(Hubei Provincial Key Laboratory for Applied Toxicology,Hubei Center for Disease Control and Prevention,Hubei Wuhan 430079,China;National Institute for Nutrition and Health,Chinese Center for Disease Control and Prevention,Beijing 100050,China)
出处
《中国食品卫生杂志》
CSCD
北大核心
2019年第4期313-319,344,共7页
Chinese Journal of Food Hygiene
基金
转基因生物新品种培育重大专项(2016ZX08011005、2014ZX08011005)
关键词
转基因小麦
大鼠
生殖发育毒性
Transgenic wheat
rats
reproductive development toxicity
