摘要
目的:探讨血清趋化因子CCL5和CXCL10用于刚地弓形虫早期诊断的价值。方法:采集44例于2013年5月-2018年5月期间在我院就诊的弓形虫症患者的血液样本,其中IgM单阳性30例,IgG单阳性10例,IgM和IgG双阳性4例。同时选取40例健康志愿者作为对照组,采用ELISA法检测各组趋化因子CCL5和CXCL10水平并采用Spearman相关性分析两趋化因子的水平相关性。此外,选取10例健康人和5例IgG阳性患者全血,抽提单个核细胞(PBMC),将5例健康人PBMC、5例患者PBMC与RH株弓形虫速殖子共培养,检测培养上清趋化因子CCL5和CXCL10水平。结果:弓形虫症患者的CCL5和CXCL10水平明显高于健康对照组(P<0.01),其中IgM单阳性组水平高于IgG单阳性组(P<0.05),且CCL5和CXCL10水平呈正相关(r=0.46,P<0.01)。健康人PBMC与弓形虫速殖子共培养分泌的CCL5和CXCL10水平较弓形虫症患者PBMC高,但差异无统计学意义。结论:血清趋化因子CCL5和CXCL10水平对弓形虫急性感染具有诊断价值,或与其在弓形虫感染进程不同阶段的释放有关。
Objective:Aim To explore the diagnostic value of serum chemokines CCL5 and CXCL10 for acute infection of Toxoplasma gondii.Methods:A total of 44 patients infected with Toxoplasma gondii,who were diagnosed in our hospital from May 2013 to May 2018.The patients were divided into 3 groups,which were IgM positive group,IgG positive group and IgM+IgG positive group.40 healthy volunteers were selected as control group.The serum chemokines were detected in each group by ELISA assays and univariate analysis between 2 chemokines were performed by Spearman correlation analysis.PBMCs from 5 healthy controls and 5 patients were infected with T.gondii and the cell supernatant chemokines CCL5 and CXCL10 were detected by ELISA assay.Results:The level of CCL5 and CXCL 10 of patients were significantly higher than those of the control group(P<0.01),and the level of IgM group were higher than those in IgG group(P<0.05).CXCL10 was positively correlated with CCL5(r=0.46,P<0.01).The level of CCL5 and CXCL10 secreted by PBMCs from health volunteers were higher than those from patients,but the difference was not significant.Conclusion:Serum levels of CCL5 and CXCL10 can assist to diagnose for T.gondii acute infection,which may be related to the function in T.gondii infection process.
作者
李文东
唐娟
卓卫
LI Wendong;TANG Juan;ZHUO Wei(.Maternal and Child Health Hospital of Huli District at Xiamen City in Fujian Province,361009)
出处
《医学理论与实践》
2019年第18期2879-2882,共4页
The Journal of Medical Theory and Practice
关键词
刚地弓形虫
急性感染
趋化因子
诊断标志物
Toxoplasma gondii
Acute infection
Chemokines
Diagnostic biomarker
