摘要
在现已优化出的马比木喜树碱提取工艺基础上,采用同一产地样品,分别以7个浓度梯度(40%~100%)的甲醇、乙醇为提取液,实验筛选出提取率最高的提取液为70%的甲醇;进一步比较了目前马比木喜树碱提取的4几种方法,提取效率值显示浸泡超声热回流提取法最优。在此基础上,利用响应面法对提取工艺进行了进一步优化,获得了最佳提取工艺为:浸泡时间14.80 h,超声时间30.70,液料比为60.00,70℃水浴中回流1.5 h。在此工艺条件下,喜树碱含量为2.29%,回归得到的模型预测效果较好。将优化工艺用于提取12个产地的马比木喜树碱含量,将其与文献报道的相同区域样品所测得最高含量比较,优化出的工艺测得含量是其他工艺的2.4至10倍,大幅度提高了喜树碱提取率,具有明显优越性。
Based on the optimized extraction process of amptothecin from Nothapodytes Pittosporoides,the same origin samples were used,and 7 concentrations of concentration(40%-100%)of methanol and ethanol were used as extracts.The extraction methanol wasmethanol and ethanol with 7 concentration gradients(40%-100%).In this paper,four extraction methods of Camptothecin from Nothapodtes Pittosporoides were compared,and the extraction efficiency showed that soak ultrasonic heat reflux extraction method was optimal.On this basis,the response surface method was used to furtherly optimize extraction process of Camptothecin from Nothapodytes Pittosporoides.The optimal extraction process was soak 14.8 h,super living time 30.70 min,solid-liquid ratio 60.00,temperature 70℃heat reflux extraction time 1.5 h.Under this process conditions,the content of Camptothecin fromNothapodytes Pittosporoides was 2.29%,and the regression model has a better prediction effect.Content of Camptothecin from 12 origin Nothapodytes Pittosporoideswere detected by using optimize process.With the literature reported the same region,the sample measured and the highest content comparison,content detection of this paper optimized process was 2.4 to 10 times of other processes.Optimized process greatly improved the camptothecin extraction yield with obvious advantages.
作者
周玮
杨艳
张凡
高渐飞
Zhou Wei;Yang Yan;Zhang Fan;Gao Jianfei(Guizhou Industry Polytechnic College,Guizhou,Guiyang,550008,China;The Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences,Guizhou,Guiyang,550002,China;Guizhou mountainous Resources Institute,Guiyang,Guizhou,55000,China)
出处
《绿色科技》
2019年第18期163-166,170,共5页
Journal of Green Science and Technology
基金
贵州省科技支撑计划项目(编号:黔科合SY字[2014]3060号)
关键词
马比木
喜树碱
响应面优化
提取工艺
Nothapodytes Pittosporoides
Camptothecin
response surface
extraction process
