摘要
试验建立了饲料中9种香味剂乙酸异戊酯、乙基麦芽酚、肉桂醛、大茴香脑、γ-壬内酯、肉桂酸甲酯、香兰素、乙基香兰素、桃醛的气相色谱测定法。结果表明,9种香味剂在5.00~500 g·mL^-1浓度范围内呈现良好的线性关系,相关系数r≥0.999,添加浓度为20、50、100、200、500、1 000 mg·kg^-1,进行加标回收试验,表明在添加上述6个不同浓度,其回收率为70.1%~117.5%,重复性RSD最大的为11.4%,具有较高的准确性和重现性。方法的检测限为20 mg·kg^-1,定量限为50 mg·kg^-1。经方法学验证,本方法灵敏度高,简便、准确、快速,适用于饲料中香味剂类药物的检测。
A gas chromatographic method was established for the determination of nine flavoring agents in feed,including isoamyl acetate,ethyl maltol,cinnamaldehyde,anise,gamma-nonyllactone,methyl cinnamate,vanillin,ethyl vanillin and peach aldehyde.The result showed that nine flavoring agents showed a good linear relationship in the concentration range of 5.00-500 g·mL^-1.The correlation coefficient r was greater than or equal to 0.999.The standard recovery test was carried out with the addition concentration of 20,50,100,200,500 and 1 000 mg·kg^-1.The results showed that the recovery rate was higher with the addition of the six different concentrations mentioned above.70.1%-117.5%and 11.4%of repetitive RSD,respectively,have high accuracy and reproducibility.The detection limit was 20 mg·kg^-1 and the quantitative limit was 50 mg·kg^-1.The method was proved to be specific,simple,accurate and rapid,and suitable for the determination of aromatics in feed.
作者
郭国贤
GUO Guoxian(Liaoning Provincial Inspection and Testing Certification Center,Liaoning Inspection and Testing Institute for Agricultural Products and Veterinary Drug Feed Products,Shenyang 110000,China)
出处
《饲料博览》
2019年第9期29-32,共4页
Feed Review
关键词
气相色谱法
饲料香味剂
测定
gas chromatography
feed flavoring agents
determination
