摘要
本研究通过林间病情观察,组织分离法分离纯化病健交界处病原菌,根据柯赫氏法则检测其致病性,采用形态学方法初步鉴定了7株典型炭疽属真菌,分别为MYGD1、MYGD2、MYGD3、MYGD4、MYGD5、MYGD6和MYGD7,结合形态学及分子鉴定对该7株病原菌进行了系统鉴定。结果表明:在ITS-CAL-GAPDH-ACT-TUB 5个基因所构建的系统发育树中,MYGD7与卡哈瓦炭疽菌(Colletotrichum kahawae)以99%的置信度聚为一个独立进化分支;MYGD1与果生炭疽菌(Colletotrichum fructicola)的权威菌株ICMP18613以99%的置信度聚为一个独立进化支;MYGD2、MYGD3、MYGD4、MYGD5、MYGD6与山茶炭疽菌(Colletotrichum camelliae)的权威菌株ICMP 10643和ICMP 18542以99%的置信度聚为一个独立进化分支。根据形态学特征,结合多基因系统发育树,鉴定MYGD7为卡哈瓦炭疽菌,MYGD1为果生炭疽菌,MYGD2、MYGD3、MYGD4、MYGD5和MYGD6为山茶炭疽菌。这是卡哈瓦炭疽菌为油茶炭疽病病原菌的首次报道。炭疽病是油茶的主要病害之一,明确贵州省油茶炭疽病的病原菌种类,对该病害的科学防治具有重要意义。
In this study, through the observation of disease in forest, the pathogenic fungi were isolated and purified by the tissue separation method, and the pathogenicity was detected according to the kirsch’s rule. Seven typical anthrax fungi were identified by morphological method, namely MYGD1, MYGD2, MYGD3, MYGD4,MYGD5, MYGD6 and MYGD7, respectively, which were systematically identified by morphological and molecular identification. The results showed that MYGD7 and Colletotrichum kahawae were clustered into an independent evolutionary branch with the confidence of 99% in the phylogenetic tree constructed by the sequence of five genes of ITS-CAL-GAPDH-ACT-TUB. The MYGD1 were group into an independent evolutionary branch including the authoritative strain ICMP18613 of Col-letotrichum fructicola, with confidence of 99%. The MYGD2, MYGD3,MYGD4, MYGD5 and MYGD6 were grouped into a cluster branch consisting of the authoritative strain ICMP10643 and ICMP 18542 of Colletotrichum camelliae, with the confidence of 99%. According to the morphological characteristics combined with evolutionary tree analysis of multigenes, the MYGD1 was identified as C. fructicola,the MYGD2, MYGD3, MYGD4, MYGD5 and MYGD6 were C. camelliae, and the MYGD7 was C. kahawae. This is the first report for C. kahawae is new pathogenic strain for anthracnose of C. oleifera. Anthracnose is one of main diseases of Camellia oleifera. Identification of kinds of anthracnose pathogen of C. oleifera in Guizhou province could provide great significance for the prevention and control of this disease.
作者
帅小春
阮成江
熊朝伟
安启明
杨明
张涛
赵艳梅
杨斯琪
马梦晗
朱元玲
Shuai Xiaochun;Ruan Chengjiang;Xiong Chaowei;An Qiming;Yang Ming;Zhang Tao;Zhao Yanmei;Yang Siqi;Ma Menghan;Zhu Yuanling(Key Laboratory of Biotechnology and Bioresources Utilization,Ministry of Education,Institute of Plant Resources,Dalian Minzu University,Dalian,116600;Forestry Bureau of Tongren City,Tongren,554300;Forestry Bureau of Yuping Dongzu Autonomous County,Yuping,556000)
出处
《分子植物育种》
CAS
CSCD
北大核心
2019年第18期5939-5945,共7页
Molecular Plant Breeding
基金
贵州省科技支撑计划项目(黔科合支撑[2017]2521
[2016]2517-1
[2016]2519)
基础研究计划(黔科合基础[2018]1159)
大学生创新创业训练计划省级项目(201812026141)共同资助
