摘要
本文以‘香野’草莓匍匐茎茎尖为试材,采用组织培养法,研究了外植体消毒灭菌、诱导分化培养、继代增殖培养、生根培养等组培快繁关键技术,建立了‘香野’草莓茎尖组织培养快繁技术。结果表明:用10%次氯酸钠对外植体消毒5 min可达到最好的消毒效果,成活率最高为64.44%。在外植体诱导分化阶段,草莓外植体诱导分化最适宜培养基配方为MS+噻苯隆(TDZ)1.0 mg/L+萘乙酸(NAA)0.1 mg/L,诱导分化率为67.78%。在丛生芽继代增殖阶段,草莓丛生芽增殖最适宜培养基配方为MS+TDZ(0.75 mg/L)+NAA(0.2 mg/L),增殖系数为4.11。在生根阶段,草莓丛生芽生根最适宜培养基配方为MS+NAA 0.1 mg/L,生根率为98.33%。
In this paper,with the stolon tip of‘Xiangye’strawberry used as test material,the key technology of rapid propagation of explants such as sterilization,induction and differentiation culture,subculture and rooting culture were studied with tissue culture method,and the rapid propagation technology of strawberry cultivar‘Xiangye’stem tip tissue culture was established.The results showed that using 10%sodium hypochlorite to disinfect the explants for 5 minutes,the disinfection effect was the best and the survival rate was the highest,reaching 64.44%.The best medium for strawberry explant induction and differentiation was MS+Thidiazuron(TDZ)1.0 mg/L+Naphthalene acetic acid(NAA)0.1 mg/L,and the induction and differentiation rate was 67.78%at the stage of explant induction and differentiation.The best medium formula for cluster bud multiplication of strawberry was MS+TDZ(0.75 mg/L)+NAA(0.2 mg/L),and the value-added coefficient was 4.11 at the stage of cluster bud subculture and multiplication.The optimum medium for rooting of strawberry cluster buds was MS+NAA 0.1 mg/L and the rooting rate was 98.33%in the rooting stage.
作者
连朋
杜梦卿
王丽娟
LIAN Peng;DU Meng-qing;WANG Li-juan(College of Horticulture and Landscape,Tianjin Agricultural University,Tianjin 300384,China)
出处
《天津农学院学报》
CAS
2019年第3期21-25,共5页
Journal of Tianjin Agricultural University
基金
天津市种业重大项目（17ZXZYNC00080）
天津市科技成果转化与推广项目（201803010）
关键词
草莓
茎尖
消毒
激素
组培
strawberry
shoot tip
disinfection
hormone
tissue culture
