摘要
目的探讨通痹活络汤煮散的煎煮工艺。方法采用高效液相色谱法(HPLC),选用XBridge C18色谱柱,以乙腈-水(49:51)为流动相,检测波长为330、230 nm,柱温为室温25℃测定通痹活络汤中蛇床子素和芍药苷的含量。以总固体和指标性成分的含量作为评价指标,采用正交试验筛选其最佳煎煮工艺。通过与传统饮片药液质量比较,筛选其煮散剂量。结果蛇床子素在0.1224~4.0800μg范围内,线性关系良好(r=0.9999),平均加样回收率为101.14%,RSD为1.42%;芍药苷在0.8194~16.3880μg范围内,线性关系良好(r=0.9999),平均加样回收率为99.55%,RSD为1.79%。最佳煎煮工艺是加12倍水,浸泡5 min,每次煎煮20 min,煎煮1次。通过筛选煮散临床推荐剂量试验,选用相当于通痹活络汤传统饮片35%的煮散剂量作为临床推荐剂量。结论优选的煎煮工艺简单快速,稳定可行,筛选的剂量可为临床应用提供指导。
Objective To explore the decoction technology of Tongbi Huoluo Decoction.Methods The content of osthole and paeoniflorin in Tongbi Huoluo Decoction were determined by high performance liquid chromatography(HPLC)with XBridge C18 column,acetonitrile-water(49:51)as mobile phase,detection wavelength at 330,230 nm and column temperature at room temperature of 25℃.The contents of total solids and index components were used as evaluation indexes,and orthogonal test was used to screen the decoction process.By comparing the quality of decoction with that of traditional decoction pieces,the dosage of decoction was screened.Results Ostholewas in the range of 0.1224-4.0800μg,with a good linear relationship(r=0.9999),the average recovery rate was 101.14%,RSD was 1.42%.Paeoniflorin has a good linear relationship in the range of 0.8194-16.3880μg(r=0.9999),with an average recovery of 99.55%and RSD of 1.79%.The best decocting process was to add 12 times of water,soak for 5 min,decocting for 20 min each time,and decocting once.Through screening the clinical recommended dose test of decoction,the decoction dose equivalent to 35%of the traditional decoction pieces of Tongbi Huoluo Decoction was selected as the clinical recommended dose.Conclusion The optimized decocting process is simple,rapid,stable and feasible,and the selected dosage can provide guidance for clinical application.
作者
朱卫星
张敏
甘国兴
龙国斌
余文新
ZHU Wei-xing;ZHANG Min;GAN Guo-xing;LONG Guo-bin;YU Wen-xin(Department of Pharmacy,Qingyuan Hospital of Traditional Chinese Medicine,Guangdong Province,Qingyuan511500,China;Engineering Technology Research Center of Fresh Medicine and National Medicine in Guangdong Province,Qingyuan511500,China;Department of Pharmacy,Guangdong Provincial Hospital of Traditional Chinese Medicine,Guangzhou510115,China)
出处
《中国当代医药》
2019年第27期7-11,共5页
China Modern Medicine
基金
全国中药特色技术传承人才培训项目
广东省清远市社会发展领域自筹经费科技计划项目(2017B046)
关键词
煮散
通痹活络汤
煎煮工艺
总固体
蛇床子素
芍药苷
Boiling and dispersing
Tongbi Huoluo Decoction
Decocting process
Total solids
Osthole
Paeoniflorin
