摘要
从麦冬根部分离内生菌,研究分离出的内生菌的抗菌活性,并对分离出的内生菌进行染色、镜检,观察其细菌形态,确定为革兰阳性菌。以金黄色葡萄球菌、大肠杆菌、枯草芽孢杆菌为指示菌,初步筛选出有抗菌活性的麦冬内生菌。培养的内生菌提取基因组,对其16S rDNA基因进行PCR扩增,电泳结果显示,从麦冬中分离出的内生菌的DNA片段大小为1500 bp左右。
Endophytic bacteria were isolated from the roots of Ophiopogon japonicus,and their antibacterial activity was studied.The isolated endophyte was stained.The morphology of the bacterium was observed under an electronic microscope,and the bacterium was determined to be gram-positive.With Staphylococcus aureus,Escherichiacoli and Bacillus subtilis as the research objects,endophytic bacteria with antibacterial activity were screened from Ophiopogon japonicus.The cultured endophyte was chosen to extract the genome,and its 16S rDNA gene was amplified by PCR.The electrophoresis results showed that the size was about 1500 bp of the DNA fragment of the endophyte isolated from Ophiopogon japonicus.
作者
连瑞丽
李宇伟
王宇静
李利红
LIAN Ruili;LI Yuwei;WANG Yujing;LI Lihong(College of Animal Medicine of Henan University of Animal Husbandry and Economy,Zhengzhou,Henan 450046,China)
出处
《现代牧业》
2019年第3期23-27,共5页
Modern Animal Husbandry
基金
河南牧业经济学院“中兽药生药学”重点培育学科项目(41000003)
河南牧业经济学院“中兽药新制剂创制”科技创新团队项目(HUAHE2015009)
河南省科技厅科技攻关项目(162102310434)
关键词
麦冬
内生菌
抗菌活性
筛选
Ophiopogon japonicus
endophytic bacterium
antibacterial activity
selection
