摘要
为研究长链脂肪酸组合对体外培养瘤胃微生物发酵挥发性脂肪酸(VFA)的影响,并得出最优脂肪酸组合及添加水平,试验选取硬脂酸、油酸、亚油酸及α-亚麻酸4种脂肪酸因子,设置0.5%、1.0%、1.5%等3个水平进行L9(3^4)正交试验,其中Ⅰ~Ⅸ组为试验组、Ⅹ组为对照组,各处理均设3个重复。以3头瘤胃瘘管山羊为瘤胃液供体进行体外发酵试验,测定各组培养液VFA 24 h内的动态变化。结果显示,各组的总挥发性脂肪酸(TVFA)浓度总体上呈先上升后下降再上升的波动变化趋势。其中在6 h时Ⅶ组TVFA浓度最高。乙酸/丙酸比值总体呈下降趋势,6 h前下降较快,6 h后趋于平缓。丁酸摩尔百分比在7.97%~15.41%间变动。主成分与最优组合分析表明,TVFA浓度影响的主次顺序为亚油酸、油酸、硬脂酸、α-亚麻酸;以添加1.5%硬脂酸、0.5%油酸、1.5%亚油酸、0.5%α-亚麻酸时组合效应最好。
In order to study the effects of long-chain fatty acid combinations of stearic acid,oleic acid,linoleic acid andα-linolenic acid(ALA)on volatile fatty acid(VFA)in vitro fermentation,and to obtain their optimal fatty acid combination and the levels of addition,the orthogonal test were conducted using L 9(3^4)orthogonal table with 4 factors(stearic acid,oleic acid,linoleic acid,ALA)and 3 levels(0.5%,1.0%,1.5%).Treatments were run in triplicate.The test groups were divided into 10 groups:Ⅰ-Ⅸ(test groups)andⅩ(control group).Three rumen fistula goats were provided with rumen fluid for in vitro culture,and the 24 h dynamics of VFA concentration were measured.The results showed as follows:The concentration of total volatile fatty acid(TVFA)in all groups fluctuated with time,and had a first upward,then down and upward again trend.At 6 h,the TVFA concentration of groupⅦwas the highest.The acetate acid/propionate acid dropped rapidly before 6 h,and leveled off after 6 h.The percentage of butyric acid molar concentration in TVFA was changed from 7.97%to 15.41%.Principal component and optimal combination analysis showed that the order affecting the TVFA concentration was linoleic acid,oleic acid,stearic acid,and ALA.The combination effect was best when 1.5%stearic acid,0.5%oleic acid,1.5%linoleic acid,and 0.5%ALA were added.
作者
习瓒娜
高健
王梦芝
XI Zanna;GAO Jian;WANG Mengzhi(College of Animal Science and Technology,Yangzhou University,Yangzhou 225009,China;China Agricultural University,Beijing 100089,China)
出处
《中国畜牧兽医》
CAS
北大核心
2019年第10期2916-2926,共11页
China Animal Husbandry & Veterinary Medicine
基金
国家自然基金项目(31672446)
国家十三五重点研发计划子课题(2018YFD0502100)
江苏省优势学科(PAPD)
