摘要
为研究铜离子暴露对中国大鲵的毒性效应,以中国大鲵幼体为研究对象,采用慢性染毒法,将大鲵幼体暴露于0 mg/L、1.0 mg/L、1.5 mg/L和3.0 mg/L的铜溶液中30 d,观察铜离子胁迫下的大鲵幼体临床症。之后立即对大鲵幼体进行解剖并观察其皮肤、肝脏、胰脏和小肠组织的病理变化,同时采集大鲵幼体的皮肤、肝脏、胰脏和小肠,利用石蜡切片和HE染色对大鲵幼体的组织病理损伤进行观察。结果表明,随着铜离子浓度的增加,大鲵幼体异常行为与其体表黏液的分泌也越加频繁;铜离子胁迫会导致大鲵幼体膀胱显著膨大并充斥大量尿液;铜离子对大鲵幼体皮肤的真皮结构、肝脏和胰脏细胞会产生一定的病理损伤,但对大鲵幼体皮肤、肝脏、胰脏和小肠组织结构未造成毒害效应。本研究表明,铜离子胁迫30 d后会对大鲵幼体产生一定的毒性效应,并且毒性程度与铜离子的浓度呈正相关关系。
In order to study the toxic effects of copper ion exposure on Chinese giant salamander,juvenile Chinese giant salamander was used as research subjects and was exposed to 0 mg/L,1.0 mg/L,1.5 mg/L and 3.0 mg/L copper ions for 30 d by adopting chronic toxicity tests.The clinical symptoms of juvenile Chinese giant salamander under copper stress were observed.And juvenile Chinese giant salamander was dissected immediately to observe the pathological changes of skin,liver,pancreas and small intestine tissue.At the same time,the skin,liver,pancreas and small intestine tissue were collected for further histopathological observations by using of hematoxylin and eosin staining of paraffin sections.The results showed that with the increase of copper ions concentration,the abnormal behavior and mucous secretion of juvenile Chinese giant salamander were more frequent.The copper ion stress caused the bladder of juvenile giant salamander enlarge significantly and was filled with a large amount of urine.The pathological damage of dermal structure of skin,the cells of liver and pancreas were caused by copper ions,while it had no toxic effects on the skin,liver,pancreas and the structure of small intestine tissue.This study showed that the stresses of copper ions had a certain toxic effect on juvenile giant salamander after 30 d,and the degree of toxicity was positively correlated with the concentration of copper ions.
作者
钱璟
耿冉
张耀武
陈万光
QIAN Jing;GENG Ran;ZHANG Yaowu;CHEN Wanguang(Research Center of Aquaculture Engineering Technology of Cold Water Fish in Henan Province,Luoyang Normal College,Luoyang,Henan,471934,China;Guangxi Key Laboratory for Marine Biotechnology,Guangxi Institute of Oceanography,Guangxi Academy of Sciences,Beihai,Guangxi,536000,China)
出处
《广西科学院学报》
2019年第3期200-205,共6页
Journal of Guangxi Academy of Sciences
基金
河南省高等学校重点科研项目（18B240002）资助
关键词
铜离子
中国大鲵
胁迫效应
组织病理学
毒性效应
解剖特征
病理变化
copper ions
Andrias davidianus
stress effects
histopathology
toxic effects
anatomical characteristics
pathological changes
