摘要
【目的】为了探究杉木在酸铝胁迫下抗氧化酶活性的变化规律及其作用机理,以此为基础探索逆境胁迫下杉木的抗性机制。【方法】通过土培盆栽试验,以1年生杉木实生苗为试验对象,采用0.24 g·kg-1的AlCl 3·6H 2O模拟铝胁迫、pH 4.0的酸液模拟酸胁迫以及两者共施模拟酸铝复合胁迫,测定幼苗叶片不同胁迫时间(15、30和45 d)的MDA含量和SOD、POD、CAT、PPO等酶活性。【结果】无酸无铝状态下,MDA含量均处于低水平状态,单酸、单铝和酸铝处理皆引发MDA的过量积累,且影响程度是酸铝复合胁迫>铝胁迫>酸胁迫。在单酸、单铝和酸铝处理下,发现POD和SOD酶活性的增长幅度是酸铝复合胁迫>酸胁迫>铝胁迫;CAT和PPO酶活性的增长幅度则是酸铝复合胁迫>铝胁迫>酸胁迫。随着胁迫时间延长,POD、SOD活性均先增后减;CAT活性在铝胁迫和酸铝复合胁迫下先增后减,酸胁迫下逐渐增加;PPO活性则均逐渐增加。【结论】单酸、单铝和酸铝处理对POD、SOD、CAT和PPO活性均产生不同程度的诱导,但同时引发MDA的积累。酸铝复合胁迫对抗氧化酶活性具有一定的协同效应,酸胁迫对POD和SOD活性的促进作用大于铝胁迫,对于CAT和PPO活性,铝胁迫的促进作用则大于酸胁迫。
【Objective】The antioxidant enzyme activities and respond mechanism of Chinese fir(Cunninghamia lanceolata)under acid and/or aluminum stresses were investigated.【Method】A pot experiment was conducted to determine the MDA content and SOD,POD,CAT,and PPO activities in one-year-old leaves of Chinese fir seedlings under the stress for 15,30 or 45 d.At the rate of 0.24 g·kg-1,AlCl 3 6H 2 O was applied to the pots to simulate an aluminum stress,a pH 4.0 acid solution added to simulate an acid stress,and the combination of the two incorporated to simulate an acid-aluminum stress.【Result】Without the presence of acid or aluminum,the potted seedlings had a low content of MDA in the leaves.However,the addition of acid,aluminum or acid-aluminum combination induced excessive accumulation of MDA with the severities ranking as acid-aluminum stress>aluminum stress>acid stress.The addition also raised the POD and SOD activities in leaves with a rank of acid-aluminum stress>acid stress>aluminum stress,and the CAT and PPO activities in the order of acid-aluminum stress>aluminum stress>acid stress.A prolonged treatment caused an initial increase on the POD and SOD activities followed by a decline;and on the CAT activity similarly under the aluminum or acid-aluminum stress but a steady increase under the acid stress;whereas,a gradual increase on the PPO activity under any of the 3 treatments.【Conclusion】The acid,aluminum or acid-aluminum stress affected the POD,SOD,CAT and PPO activities in the leaves to varying extents and raised MDA accumulation in the leaves of Chinese fir seedlings.There appeared to be a synergistic effect on the activities between acid and aluminum.But alone,the acid stress exerted a greater effect on POD and SOD than did aluminum,whereas,aluminum seemed to be a more formidable adversary than acid for the seedlings on CAT and PPO.
作者
王培
张家君
刘翠
马志慧
陈宇
林思祖
WANG Pei;ZHANG Jia-jun;LIU Cui;MA Zhi-hui;CHEN Yu;LIN Si-zu(College of Forestry,Fujian Agriculture and Forestry University,Fuzhou,Fujian350002,China;State Forestry Administration Engineering Research Center of Chinese Fir,Fuzhou,Fujian350002,China;College of Life Sciences,Fujian Agriculture and Forestry University,Fuzhou,Fujian350002,China)
出处
《福建农业学报》
CAS
CSCD
北大核心
2019年第7期852-857,共6页
Fujian Journal of Agricultural Sciences
基金
国家林业局杉木工程技术研究中心平台建设项目(ptjh130002),国家林业局杉木工程技术研究中心孵化基金(6213C011103)
关键词
杉木
酸铝
抗氧化酶活性
MDA
Chinese fir
acid-aluminum stress
oxidase activity
MDA
