摘要
为探究内生细菌对宿主植物光合作用的影响机制,以雷公藤(Tripterygiun wilfordii)组培苗为材料,研究了单独和混合接种内生细菌对光合作用参数和叶绿素荧光参数的影响。结果表明,接种菌株LY3+LJ3、LG6+LY3、LJ3+LJ12及LJ3显著提升了净光合速率(Pn)(P<0.05),相比对照分别提高了81.6%、71.4%、67.3%和18.4%,而其余接种处理则降低了Pn或无明显影响。接种LG6+LY3的气孔导度(Gs)和蒸腾速率(Tr)显著下降(P<0.05),光系统潜在活性(Fv/F0)则显著上升(P<0.05)。接种LJ3+LJ12的情况相反,Gs、胞间CO2浓度(Ci)和Tr显著升高(P<0.05),最大光化学效率(Fv/Fm)、Fv/F0和最大荧光(Fm)则显著下降(P<0.05);接种LY3+LJ3显著升高了Gs和Tr(P<0.05),其余指标无明显变化。与单独接种相比,混合接种LG6+LY3、LJ3+LJ12、LY3+LJ3对宿主光合作用具有协同促进作用。可见,不同的内生菌对光合作用的影响机制不同,LG6+LY3主要通过调节叶绿素荧光改善光合速率,气孔因素的改善是LJ3+LJ12提升光合速率的主要原因。
In order to explore the mechanism of endophytic bacteria affecting the photosynthesis of host plants,the effects of single and combined inoculation with endophytic bacteria on photosynthesis and chlorophyll fluorescence parameters were studied by using Tripterygium wilfordii tissue culture seedlings.The results showed that inoculations with strains,LY3+LJ3,LG6+LY3,LJ3+LJ12 and LJ3 significantly enhanced the net photosynthetic rate(Pn)(P<0.05),which were 81.6%,71.4%,67.3%and 18.4%higher than that of the control,respectively;while the other inoculation treatments reduced Pn or had no significant effect.When co-inoculated with LG6+LY3,the stomatal conductance(Gs)and the transpiration rate(Tr)significantly declined(P<0.05),while the potential activity of PSII(Fv/F0)increased significantly(P<0.05).Oppositely,co-inoculated with LJ3+LJ12,Gs,the intercellular CO2 concentration(Ci)and Tr increased significantly(P<0.05),while the maximum photochemical efficiency(Fv/Fm),Fv/F0 and the maximum fluorescence(Fm)decreased significantly(P<0.05).Co-inoculation with LY3+LJ3 enhanced Gs and Tr(P<0.05),and had no significant influence on the other indicators.Compared with the single inoculation,co-inoculation with LG6+LY3,LJ3+LJ12 and LY3+LJ3 indicated synergistic promoting effects on the host photosynthesis.In conclusion,the endophytic bacteria showed different influence mechanism on the photosynthesis.Strains LG6+LY3 improved photosynthetic rate mainly by regulating chlorophyll fluorescence;the improvement of stomatal factors was the major reason for LJ3+LJ12 to enhance photosynthetic rate.
作者
禹胜男
孙睿
宋萍
封磊
吴承祯
洪伟
刘洁
袁梦
YU Sheng-nan;SUN Rui;SONG Ping;FENG Lei;WU Cheng-zhen;HONG Wei;LIU Jie;YUAN Meng(College of Forestry,Fujian Agriculture and Forestry University,Fuzhou 350002,China;College of Resources and Environment,Fujian Agriculture and Forestry University,Fuzhou 350002,China;Fujian Provincial Key Laboratory of Forest Ecosystem Processing and Management,Fuzhou 350002,China;College of Ecology and Resource Engineering,Wuyi University,Nanping,Fujian 354300,China)
出处
《江西农业大学学报》
CAS
CSCD
北大核心
2019年第5期963-968,共6页
Acta Agriculturae Universitatis Jiangxiensis
基金
国家自然科学基金项目(31000264,31470577)
福建省高校产学合作科技重大项目(2014N5005)~~
关键词
雷公藤
内生细菌
叶绿素荧光
光合作用
混合接种
Tripterygium wilfordii
endophytic bacteria
photosynthesis
chlorophyll fluorescence
combined inoculation
