摘要
黄萎病是降低棉花产量和品质较为严重的维管束病害。棉花在抵抗病原菌的过程中,抗病基因起着尤为重要的作用。漆酶是一种多功能氧化酶,在植物木质素合成和提高植株抗逆性等方面发挥着重要作用。高质量版本的基因组是提升基因家族分析准确性的必要条件。本研究以目前最新的陆地棉TM-1高质量版本基因组为参考,通过生物信息学分析鉴定了陆地棉基因组中的Laccase(GhirLAC)基因家族,并对其进行了理化性质、基因结构、染色体定位以及在黄萎病胁迫下的表达模式分析。结果表明,陆地棉TM-1基因组中共包含83个GhirLAC家族成员,分布在24条染色体上,所有GhirLAC蛋白均定位在胞外,且具有相同/相似的保守基序。系统发育树分析显示,GhirLAC基因家族成员可分为7个亚组。结合黄萎病胁迫下棉花转录组数据,将GhirLAC基因家族各成员的表达分为3种模式,其中第1类和第2类GhirLAC基因在黄萎病菌胁迫下分别呈现有规律的表达量升高和降低,推测这些基因在棉花抗黄萎病反应中发挥重要功能。荧光定量PCR结果表明,GhirLAC02(GhLAC4)、GhirLAC38(GhLAC11)和GhirLAC20(GhLAC12)3个候选基因均受黄萎病菌诱导表达,其表达趋势与转录组数据相吻合。本研究为以后深入解析棉花GhirLAC基因的抗病功能及分子机制奠定基础。
Verticillium dahliae stress causes a disease in vascular bundle that decreases cotton yield and fiber quality.During cotton defense against pathogen infection,disease resistance genes play important roles.Laccase is a multifunctional oxidase that plays an important role in lignin synthesis and plant resistance.High-quality cotton reference genome is necessary to improve the accuracy of gene family analysis.In this study,the laccase(GhirLAC)family genes in the update genome of G.hirsutum L.cv.TM-1 were identified by bioinformatics,and its physical and chemical properties,gene structure,chromosome location and expression pattern under V.dahliae stress were analyzed.There were 83 members of GhirLAC family in the genome of G.hirsutum L.,which distributed on 24 chromosomes.All GhirLAC proteins predicted were located in extracellular and had the same conserved motif.Phylogenetic analysis showed that the members of the GhirLAC genes family were divided into seven subgroups.According to the analysis results of cotton transcription under V.dahliae stress,it was clear that the expression pattern of GhirLAC genes could be divided into three groups,of which,group 1 and group 2 GhirLAC genes displayed down-regulation and up-regulation expression patterns,respectively,suggesting that these genes should play important roles in cotton Verticillium wilt resistance.Furthermore,we identified three candidate genes expression patterns induced by V.dahliae,including GhirLAC02(GhLAC4),GhirLAC38(GhLAC11),and GhirLAC20(GhLAC12),the qPCR results were consistent with the expression trend based on transcriptome data.This study lays a foundation for further analysis of disease resistance function and molecular mechanism of GhirLAC gene in cotton.
作者
赵晶
李旭彤
梁学忠
王志城
崔静
陈斌
吴立强
王省芬
张桂寅
马峙英
张艳
ZHAO Jing;LI Xu-Tong;LIANG Xue-Zhong;WANG Zhi-Cheng;CUI Jing;CHEN Bin;WU Li-Qiang;WANG Xing-Fen;ZHANG Gui-Yin;MA Zhi-Ying;ZHANG Yan(Hebei Agricultural University/North China Key Laboratory for Crop Germplasm Resources of Education Ministry/Co-Innovation Center for Cotton Industry of Hebei Province,Baoding 071001,Hebei,China)
出处
《作物学报》
CAS
CSCD
北大核心
2019年第12期1784-1795,共12页
Acta Agronomica Sinica
基金
河北省优秀青年科学基金项目(C2017204011)
河北省高等学校科学技术研究重点项目(ZD2014019)
河北省青年拔尖人才支持计划资助~~
关键词
陆地棉
漆酶
基因家族
黄萎病
基因表达
G.hirsutum L.
laccase
gene family
Verticillium wilt
gene expression
