摘要
为优化水提醇沉法提取油茶(Camellia oleifera)饼粕多糖工艺条件,在单因素试验基础上,选择料液比、提取温度、提取时间为自变量,油茶多糖提取率为响应值,利用Box-Behnken中心组合试验和响应面分析法,研究各自变量交互作用及其对油茶多糖提取率的影响。结果表明,油茶多糖提取最佳提取工艺为料液比1:25(g/mL)、提取时间78 min、提取温度60℃,在此条件下油茶多糖提取率为(11.29±0.20)%,与预测值偏差0.18个百分点,因此优化后回归方程对预测水提醇沉法制备油茶多糖的提取率分析具有可靠性,三因素中料液比具有显著性影响。
In order to optimize the water extraction and alcohol precipitation method for preparation of Camellia oleifera cake polysaccharides,on the basis of single factor experiment,the ratio of material to liquid,extraction temperature and extraction time were chosen as independent variables,while the extraction rate of C.oleifera polysaccharides as corresponding values.With the Box-Behnken center combined test and response surface analysis,the interaction of each independent variable and its effect on the extraction rate of C.oleifera polysaccharide were studied.The results showed that the optimum for C.oleifera polysaccharides preparation by water extraction and alcohol precipitation were 1:25(g/mL)of the ratio of solid to liquid,78 min of the extraction time,and 60°C of the extraction temperature.Under these conditions,the extraction rate of C.oleifera polysaccharides was(11.29±0.20)%,with a deviation of 0.18 percentage points from the predicted value.Therefore,this optimized regression equation has the reliability of predicting the extraction rate of C.oleifera polysaccharides by the water extraction and alcohol precipitation method,and the ratio of solid to liquid has a significant effect in the three factors.
作者
张盟雨
王静
张应中
王明怀
徐煲铧
唐旭晓
ZHANG Mengyu;WANG Jing;ZHANG Yingzhong;WANG Minghuai;XU Baohua;TANG Xuxiao(Guangdong Provincial Key Laboratory of Silviculture,Protection and Utilization/Guangdong Academy of Forestry,Guangzhou,Guangdong 510520,China)
出处
《林业与环境科学》
2019年第5期1-6,共6页
Forestry and Environmental Science
基金
广东省林业科技创新项目“油茶饼粕多糖产品开发(2017KJCX003)”
广东省级科技计划项目“广东省本土优质高产油茶良种选育研究(2015B020202002)”和“云浮市特色林果产业科技创新服务中心建设(2016A040401008)”
关键词
油茶饼粕多糖
水提醇沉法
提取率
响应面分析
Camellia oleifera cake polysaccharides
water extraction and alcohol precipitation
extraction rate
response surface methology
