摘要
为建立18个不同产地菜籽油样品的高效液相指纹图谱。本文采用HPLC-DAD检测,用Hypersil ODS C18色谱柱(4.6 mm×250 mm,5μm),流动相为乙腈和0.1%磷酸(梯度洗脱),流速1 mL/min,进样量为10μL,在280 nm波长下,测定18个不同产地菜籽油样品。采用《中药色谱指纹图谱相似度评价系统(2004年A版)》,以S18为对照指纹图谱计算得出18个油样品的相似度。18个油样品的HPLC-DAD指纹图谱有23个共有峰,除S9、S13、S15、S18外,其他油样品的相似度均大于0.8。本实验方法重现性好,具有一定的专属性,为不同地区菜籽油的初步鉴别和质量评价提供了参考。
To establish the HPLC fingerprints of rapeseed oil samples from 18 different habitats.18 different habitats were detected on Hypersil ODS C18 column(4.6×250 mm,5μm)with mobile phase consisted of acetonitrile-0.1%phosphate solution(gradient elution)at the flow rate of 1 mL/min.The chromatographic conditions by HPLC-DAD were the injection volume of 10μL and the wavelength of 280 nm.Similarity of 18 samples was computed to the peak of S18 by using software of Similarity Evaluation System for Chromatographic Fingerprint of TCM(Version 2004 A).The HPLC-DAD fingerprints of 18 rapeseed oil samples have 23 stable common peaks.The similarity of oil samples larger than 0.8 except S9、S13、S15、S18.The experimental method has good reproducibility and certain specificity.And it provides a new method to evaluate the quality and preliminary identification of rapeseed oil.
作者
华姝雯
王磊
饶桂维
Hua Shuwen;Wang Lei;Rao Guiwei(College of Biology and Environmental Engineering,Zhejiang Shuren University,Hangzhou 310015,China;Polytechnical Institute of Qianjiang College,Hangzhou Normal University,Hangzhou 310018,China)
出处
《山东化工》
CAS
2019年第20期101-105,共5页
Shandong Chemical Industry
基金
浙江省高校实验室工作研究项目(YB201845)
关键词
菜籽油
高效液相指纹图谱
相似度分析
共有峰面积
rapeseed oil
high performance liquid chromatography fingerprint
similarity evaluation
peak area of the common peak
