摘要
目的初探黑蒜对急性酒精性肝损伤的保护作用及其机制。方法小鼠分5组,用高和低浓度黑蒜溶液、生理盐水(阴性对照)、水飞蓟素片溶液(阳性对照)分别灌胃一周,采用50%酒精灌胃的方法构建小鼠的急性酒精性肝损伤模型,另有一组小鼠以生理盐水灌胃一周后不建立模型,为空白对照。各组小鼠取血清测定ALT、SOD活性及取肝切片观察组织学改变,将实验组与各对照组小鼠对比,以初探黑蒜对急性酒精性肝损伤的保护作用及其机制。结果阴性对照与空白对照相比,小鼠血清ALT水平显著升高(P≤0.01),肝切片见明显组织损伤,SOD活性明显降低(P≤0.05)。高剂量黑蒜实验组的血清ALT较阴性组有显著降低(P≤0.01),肝切片可见组织损伤程度显著降低,SOD活性明显升高(P≤0.05)。结论50%酒精灌胃可建立小鼠急性酒精性肝损伤模型,高浓度黑蒜通过抗氧化对小鼠急性酒精性肝损伤发挥保护作用。
OBJECTIVE To explore whether black garlic has preventive and protective effect against acute alcoholic hepatic injury and its mechanism,our group conducted an experiment in vivo.METHODS Mice were divided into 5 groups(blank control group,negative control group,positive control group,black garlic at low and high concentration groups)and gavaged for 7 days.50%ethyl alcohol was chosen to set up model of acute hepatic injury.Content of ALT and T-SOD in blood of ice along with histological sections in each group were measured and analysed.RESULTS Compared with blank control group without alcohol,negative control group showed significant rise in ALT(P≤0.01)and sharp drop in SOD(P≤0.05).Compared with negative control group,which gavaged with normal saline,black garlic at high concentration could reduce the ALT significantly(P≤0.01).Histological sections showed the same result.CONCLUSION 50%ethyl alcohol can set up the model of acute hepatic injury in mice;black garlic at high concentration has protective effect against acute alcoholic hepatic injury through antioxidation.
作者
张远
许彬欣
孙伟
钟烨文
袁玲玲
赵桂溢
杨再吴
郑烨新
张家强
伍宇平
杨惠玲
ZHANG Yuan;XU Bin-xin;SUN Wei;ZHONG Ye-wen;YUAN Ling-ling;ZHAO Gui-yi;YANG Zai-wu;ZHENG Ye-xin;ZHANG Jia-qiang;WU Yu-ping;YANG Hui-ling(Expermental center for basic medical teaching,Sun Yat-sen University,Guangzhou 510030,China;Department of pathophysiology,Zhongshan School of Medicine,Sun Yat-sen University,Guangzhou 510030,China)
出处
《海峡药学》
2019年第10期24-27,共4页
Strait Pharmaceutical Journal
关键词
黑蒜
小鼠急性肝损伤
保护
抗氧化
Black garlic
Alcoholic hepatic injury
Protect
Antioxidation
