摘要
SBP基因家族是一类植物特有的转录因子,并与植物多种生长发育和逆境响应的基因表达调节相关。本研究从耐旱玉米自选系X923-1中克隆了一个与干旱胁迫响应相关的SBP基因,即ZmSPL16。该基因编码序列(coding sequence, CDS)全长3.3 kb,编码1 113个氨基酸。蛋白质比对发现,其编码转录因子的保守区域的锌指结构中发生了部分重复,因而推测其可能与其抗旱能力的产生相关。通过定量PCR (quantitative polymerase chain reaction, qPCR)的方法分析发现ZmSPL16在玉米根中的表达量受干旱胁迫时显著上调。通过农杆菌介导法将ZmSPL16导入到烟草基因组中,成功获得了过量表达ZmSPL16的转基因烟草。对T1代转基因烟草的抗旱性分析显示ZmSPL16的表达能够显著提高转基因烟草的抗旱性。这些结果表明,ZmSPL16是玉米耐受干旱胁迫的优异基因资源,对其进一步研究玉米抗旱育种具有十分重要的意义。
SBP family is a plant-specific transcription factor and involved in the gene expression regulation in a variety of processes in plant growth and development and stress response. In this study, a SBP gene associated with drought stress response, ZmSPL16, was cloned from drought-tolerant maize line X923-1. The CDS of ZmSPL16 is approximately 3.3 kb in length and encodes 1 113 amino acids. Protein alignment showed that a partial duplication is found in the conserved zinc-finger domain, and it was speculated to be related to the ability to drought resistance.Quantitative PCR method analysis illustrated that the expression level of ZmSPL16 in maize root was significantly up-regulated in drought stress. The ZmSPL16 was introduced into tobacco genome by Agrobacterium-mediated transformation method and ZmSPL16 overexpressing transgenic tobacco plants were successfully obtained. Drought resistance of T1 transgenic tobacco plants was evaluated and results showed that the expression of ZmSPL16 could significantly improve drought tolerance. These results indicate that ZmSPL16 is an excellent genetic resource in maize to drought tolerance, and it is of great significance for further study of drought resistance breeding in maize.
作者
崔扬
冯彦辉
陈众峰
王晓敏
王云鹏
杜贤章
Cui Yang;Feng Yanhui;Chen Zhongfeng;Wang Xiaomin;Wang Yunpeng;Du Xianzhang(Jilin Hongxiang Agriculture Corporation Hongxiang Seedling Limited Company,Changchun,130000;Laboratory of Plant Bioreactor and Genetics Engineering,Institute of Agricultural Biotechnology,Jilin Provincial Key Laboratory of Agricultural Biotechnology,Jilin Academy of Agricultural Sci ences,Changchun,130033)
出处
《分子植物育种》
CAS
CSCD
北大核心
2019年第20期6583-6589,共7页
Molecular Plant Breeding
基金
国家自然基金项目(31501235)
科技创新人才培育计划优秀青年人才基金项目(20170520075JH)
Trans生命科学梦想基金(Trans-DFLSci-001)共同资助
