摘要
评估淋巴瘤细胞MC/CAR、HUT78和RAMOS的CD52抗原呈现稳定性,并比较三种细胞用于抗CD52单抗活性检测中的优劣性。采用免疫荧光法检测淋巴瘤细胞MC/CAR、HUT78和RAMOS的CD52抗原呈现率,分析MC/CAR、HUT78和RAMOS传代培养5~20代CD52抗原呈现稳定性。分别以MC/CAR、HUT78和RAMOS作为抗CD52单抗结合活性和补体依赖细胞毒性的靶细胞进行检测,并比较其优劣性。结果显示,MC/CAR、RAMOS和HUT78的CD52抗原呈现率分别为95.5%、63.2%和38.3%。MC/CAR传代培养5~20代CD52抗原呈现率均大于90%。RAMOS传代培养5~13代CD52抗原呈现率介于60%~67%,第14~15代CD52抗原呈现率介于50%~60%,第16~20代细胞CD52抗原呈现率介于40%~50%。HUT78传代培养5~20代CD52抗原呈现率介于26.7%~38.9%。淋巴瘤细胞MC/CAR在抗CD52单抗的结合活性检测中呈现出更好的剂量依赖曲线。淋巴瘤细胞RAMOS在抗CD52单抗的补体依赖细胞毒性检测中呈现出更好的剂量效应曲线。CD52抗原呈现率方面MC/CAR>RAMOS>HUT78。结果表明,MC/CAR更适用于抗CD52单抗的结合活性的检测,RAMOS适用于抗CD52单抗的补体依赖细胞毒性检测。
To evaluate the appearance stability of CD52 antigen in lymphoma cells MC/CAR,HUT78 and RAMOS,and to compare the advantages and disadvantages of the three cells used in the biological activity detection of anti-CD52 monoclonal antibody,the appearance rate of CD52 antigen in three cells were detected by immunofluorescence.The appearance stability of CD52 antigen in MC/CAR,HUT78 and RAMOS were analyzed for 5~20 generations.The bind-ing activity and complement dependent cytotoxicity of anti-CD52 monoclonal antibody were performed using MC/CAR,HUT78 and RAMOS as target cells,respectively,and their advantages and disadvantages were compared.CD52 anti-gen appearance rate of MC/CAR,HUT78 and RAMOS were 95.5%,63.2%,and 38.3%,respectively.CD52 anti-gen appearance rates of MC/CAR were all>90%at 5~20 generations.CD52 antigen appearance rate of RAMOS was between 60%and 67%at 5~13 generations,between 50%and 60%at 14~15 generations,and between 40%and 50%at 16~20 generations.CD52 antigen appearance rate of HUT78 was between 26.7%and 38.9%at 5~20 generations.MC/CAR showed a better dose-dependent curve in binding activity of anti-CD52 monoclonal antibodies.RAMOS showed a better dose-response curve in complement dependent cytotoxicity activity of anti-CD52 monoclonal antibody.The appearance rate of CD52 antigen in lymphoma cells were MC/CAR>RAMOS>HUT78.MC/CAR was more suit-able for the binding activity detection of anti-CD52 monoclonal antibody.RAMOS was more suitable for complement de-pendent cytotoxicity detection of anti-CD52 monoclonal antibody.
作者
秦海艳
陈继军
安晨
熊颖
叶星
南建军
毛晓燕
QIN Haiyan;CHEN Jijun;AN Chen;XIONG Ying;YE Xing;NAN Jianjun;MAO Xiaoyan(Fourth Department of Research,Lanzhou Institute of Biological Products Co.,Ltd.,Lanzhou 730046,Gansu,China)
出处
《生物资源》
CAS
2019年第5期439-444,共6页
Biotic Resources
基金
兰州市科技重大专项(2017-2-1)
