摘要
目的研究八角莲饮片的质量控制新标准。方法用HPLC双波长法同时检测八角莲饮片中鬼臼毒素、4′-去甲基鬼臼毒素、山柰酚和槲皮素。确定了360 nm作为槲皮素和山柰酚的定量波长,290 nm作为鬼臼毒素与4′-去甲基鬼臼毒素的定量波长;柱温:30℃;流速:1.0 mL·min^-1;进样量:10μL。结果HPLC双波长法可以同时测定八角莲饮片中的4种成分。鬼臼毒素、4′-去甲基鬼臼毒素、山柰酚和槲皮素的线性范围分别为0.74~3.68,2.40~12.02,0.21~1.08和0.26~1.28μg;回收率分别为99.56%,100.22%,100.67%和100.44%;含量分别为9.46~31.53,0.64~12.08,1.78~2.55和1.10~2.45 mg·g^-1。结论该方法快速、简捷、稳定可靠,适用于八角莲中木脂素类和黄酮类化学成分的测定。同时,该结果也表明各批次间黄酮类成分的差异较木脂素类成分小。
Objective To study new methods for the quality control of Dysosma versipellis.Methods By using dual-wavelength detection,HPLC was used to simultaneously determine the contents of podophyllotoxin,4′-demethyl podophyllotoxin,kaempferol and quercetin in Dysosma versipellis slices,in which 360 nm as the quantitative wavelength for quercetin and kaempferol,290 nm as the quantitative wavelength for 4′-demethyl podophyllotoxin and podophyllotoxin.The column temperature was 30℃,the flow rate was 1.0 mL·min^-1,and the sample injection was 10μL.Results Podophyllotoxin,4′-demethyl podophyllotoxin,kaempferol and quercetin showed good linearity in the range of 0.74-3.68,2.40-12.02,0.21-1.08 and 0.26-1.28μg;average recovery rate were 99.56%,100.22%,100.67%and 100.44%.The contents were 9.46-31.53,0.64-12.08,1.78-2.55 and 1.10-2.45 mg·g^-1.Conclusion The method is fast,simple,stable,reliable and can be used for the determination of lignans and flavonoids.At the same time,the results also showed that the difference of flavonoids in different batches was smaller than that of lignans.
作者
胡琨
陈伟
成彦
HU Kun;CHEN Wei;CHENG Yan(Nanjing Hospital Affiliated to Nanjing University of Chinese Medicine,Nanjing 215000,China;Suzhou Hospital Affiliated to Nanjing University of Chinese Medicine,Suzhou 215009,China)
出处
《西北药学杂志》
CAS
2019年第6期711-714,共4页
Northwest Pharmaceutical Journal
基金
国家自然科学基金青年科学基金项目(编号:81704005)
南京市青年人才依托项目(编号:QNRC2016252)
关键词
八角莲
山柰酚
HPLC法
双波长
Dysosma versipellis
kaempferol
HPLC
dual-wavelength
