摘要
目的探究香菇多糖对神经胶质瘤SHG-44细胞增殖、周期、凋亡及迁移的影响。方法 CCK-8法分析香菇多糖对SHG-44细胞增殖的影响,采用流式细胞术检测香菇多糖对SHG-44细胞的周期变化,DAPI染色法结合显微镜分析观察药物对细胞凋亡形态的影响,Annexin V-FITC/PI双染法检测作用后SHG-44细胞的凋亡情况,采用细胞划伤愈合实验初步判定香菇多糖对SHG-44细胞迁移能力的影响。结果香菇多糖可以明显抑制SHG-44细胞增殖,作用48 h效果最好,IC50值为2.295 mg/mL。与0 mg/mL香菇多糖组比较,2.295 mg/mL香菇多糖能够显著抑制SHG-44细胞迁移(P<0.01),并可能诱导SHG-44细胞凋亡;并且4 mg/mL香菇多糖将SHG-44细胞周期阻滞在G0/G1期(P<0.05,P<0.01)。结论香菇多糖对人神经胶质瘤SHG-44细胞具有显著的增殖抑制作用,作用机制是通过调控SHG-44细胞周期,从而抑制细胞增殖,同时抑制肿瘤细胞迁移。
AIM To investigate the effects of lentinan(LNT) on proliferation, cell cycle, apoptosis and migration of glioma SHG-44 cells. METHODS The effects of LNT on SHG-44 cells proliferation was analyzed by CCK-8, on cell cycle by flow cytometry, on apoptotic morphology by combinative use of DAPI staining and microscopy, and on cell apoptosis by Annexin V-FITC/PI double staining, respectively. Subsequently, impact on the cellular migration ability due to LNT was preliminarily determined by using the cell scratch healing experiment.RESULTS LNT significantly inhibited the activity of SHG-44 cells, gaining the most satisfying effect at 48 h, and 2.295 mg/mL IC50 value. Compared with the group dosed with 0 mg/mL LNT, the group intervened with 2.295 mg/mL LNT shared significantly inhibited migration of SHG-44 cells(P<0.01), and inducted apoptosis of SHG-44 cells. Moreover, LNT group(4 mg/mL) displayed blocked cell cycle of SHG-44 at G0/G1 phase(P<0.05, P<0.01).CONCLUSION LNT has a significant inhibitory effect on the proliferation of human glioma SHG-44 cells, and the underlying mechanism may be the regulation of cell cycle of SHG-44 cells, thus inhibition of both the proliferation and the migration of tumor cells.
作者
万茜淋
任雨贺
吕瑞娜
李玉
陈长宝
刘淑莹
WAN Xi lin;REN Yu he;LüRui na;LI Yu;CHEN Chang bao;LIU Shu ying(Jilin Ginseng Academy,Changchun University of Chinese Medicine,Changchun 130117,China;Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi,Jilin Agricultural University,Changchun 130118,China;Institute of Special Animal and Plant Science,Chinese Academy of Agricuttural Sciences,Changchun 130112,China;Changchun Institute of Applied Chemistry,Chinese Academy of Sciences,Changchun 130022,China)
出处
《中成药》
CAS
CSCD
北大核心
2019年第11期2614-2619,共6页
Chinese Traditional Patent Medicine
基金
国家自然科学基金面上项目(21475012)
吉林省中药管理局科技项目(2018026)
吉林省人参科学研究院种子基金(2019)
吉林省自然科学基金主题引导项目(20190201297JC)
