摘要
目的了解黑龙江省边境地区蜱类携带病原体情况。方法2009—2018年分别在黑龙江省伊春市、大兴安岭地区、佳木斯市采集蜱虫标本,共处理2530只蜱虫,其中全沟硬蜱800只,森林革蜱1730只。采用real time RT-PCR法对采集的全部蜱虫开展蜱传脑炎病毒(tick-borne encephalitis virus,TBEV)、发热伴血小板减少综合征病毒(severe fever with thrombocytopenia syndrome virus,SFTSV)、鄂木斯克出血热病毒(omsk hemorrhagic fever virus,OHFV)、兰格特病毒(langat virus,LGTV)、波瓦生病毒(powassan virus,POWV)检测,采用PCR法对采集自佳木斯地区的蜱虫开展伯氏疏螺旋体(borrelia burgdorferi sensulato,B.b.s.l)、斑点热群立克次体(spotted fever group rickettsia,SFGR)检测。结果全部的蜱类标本中蜱传脑炎病毒、发热伴血小板减少综合征病毒、鄂木斯克出血热病毒、兰格特病毒、波瓦生病毒的检测结果均为阴性。佳木斯地区斑点热群立克次体和伯氏疏螺旋体,检出率分别为59.5%和8.9%。结论黑龙江省边境地区蜱虫中斑点热群立克次体和伯氏疏螺旋体携带率高。
Objective To understand the situation of ticks carrying pathogens in border areas of Heilongjiang province.Methods From 2009 to 2018,tick specimens were collected in Yichun,Daxing′anling area and Jiamusi in Heilongjiang province.A total of 2530 ticks were studied,including 800 Ixodes persulcatus and 1730 Dermacentor silvarum.Tick-borne encephalitis virus(TBEV),severe fever with thrombocytopenia syndrome virus(SFTSV),omsk hemorrhagic fever virus(OHFV),langat virus(LGTV),powassan virus(POWV)were detected by real-time RT-PCR.Spotted fever group rickettsia(SFGR)and Borrelia burgdorferi sensulato(B.b.s.l)were detected by PCR in ticks collected from Jiamusi area.Results All tick speciments collected were negative for TBEV,SFTSV,OHFV,LGTV and POWV.Tick specimens from Jiamusi carried SFGR and B.b.s.l.with positive rates of 59.5%and 8.9%.Conclusions The ticks in border areas of Heilongjiang province carry spotted fever group rickettsia and Borrelia burgdorferi,and the carrying rate of spotted fever group rickettsia is high.The monitoring and control of ticks and tick-borne diseases should be strengthened.
作者
王倩莹
刘凤明
付士红
李樊
滕世荣
陈晓菁
殷启凯
王东强
刘文婧
雷雯雯
何英
梁国栋
李浩
包名家
王环宇
Wang Qianying;Liu Fengming;Fu Shihong;Li Fan;Teng Shirong;Chen Xiaojing;Yin Qikai;Wang Dongqiang;Liu Wenjing;Lei Wenwen;He Ying;Liang Guodong;Li Hao;Bao Mingjia;Wang Huanyu(National Institute for Viral Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,China;Chinese Center for Disease Control and Prevention,Beijing 102206,China;Jiamusi Center for Disease Control and Prevention,Jiamusi 154007,China;Huanan Center for Disease Control and Prevention,Huanan 154400,China)
出处
《中华实验和临床病毒学杂志》
CAS
CSCD
2019年第5期504-508,共5页
Chinese Journal of Experimental and Clinical Virology
基金
国家科技重大专项(2018ZX10711001、2018ZX10101-002)
传染病预防控制国家重点实验室重点项目(2015SKLID505)
国家重点研发计划(2017YFC1200505)。
