摘要
【目的】丝裂原活化蛋白质激酶激酶激酶(Mitogen-activated protein kinase kinase kinase,MAPKKK)家族在植物的胁迫反应和发育过程中起重要调控作用。本研究旨在筛选雷蒙德氏棉MAPKKK基因并分析其功能。【方法】以已鉴定的拟南芥MAPKKK蛋白序列为种子序列,在已发表的雷蒙德氏棉全基因组数据库中,通过本地BLAST以及Pfam和SMART鉴定雷蒙德氏棉MAPKKK基因家族成员;采用MEGA5、GSDS在线工具以及Mapchart进行进化树、基因结构及染色体定位分析;利用已有的陆地棉芯片数据进行响应逆境胁迫和纤维不同发育时期的表达谱分析。【结果】系统鉴定了114个雷蒙德氏棉MAPKKK家族基因,根据基因结构及进化树分析分为Raf、ZIK和MEKK三个亚家族。染色体定位表明,该基因家族广泛分布于13条染色体上,并存在基因复制。与最近公布的78个雷蒙德氏棉MAPKKK家族基因相比对,获得序列完全相同的基因47个。【结论】上述研究结果有助于了解雷蒙德氏棉MAPKKK基因家族的进化与功能,为后续研究棉花乃至棉属MAPKKK基因的功能奠定基础。
[Objective]The MAPKKK gene family plays an important regulating role in response to multiple abiotic stresses and the development of plant.This study aims to identify MAPKKK genes of Gossypium raimondii and analyze their functions.[Method]In this study,based on G.raimondii genome database and bioinformatics method,G.raimondii MAPKKK family genes were identified and analyzed.Using the MEGA5,GSDS and Mapchart program,the phylogenetic tree,gene structure and chromosomes location analyses were accomplished.Based on the existing microarray data in cotton and comparative profiles of these MAPKKK genes,different expression of them in multiple abiotic stresses and the expression at different cotton fiber developmental stages were analyzed.[Result]A sum of 114 MAPKKK genes were identified systematically in G.raimondii and classified into 3 subfamilies(Raf,ZIK and MEKK)according to the gene stucture and phylogenetic tree analyses.They were distributed on all the 13 chromosomes of G.raimondii,and segmental duplication and tandem duplication events may have occurred.Compared with the recently released 78 genes of G.raimondii MAPKKK family genes,47 sequences are exactly the same ones.[Conclusion]The results are helpful to understand the evolution and function of MAPKKK gene family.Our results provide a foundation for future functional characterizations of MAPKKK genes in cotton and probably other Gossypium plants.
作者
许好标
李黎贝
张驰
冯震
喻树迅
Xu Haobiao;Li Libei;Zhang Chi;Feng Zhen;Yu Shuxun(State Key Laboratory of Subtropical Silviculture/Zhejiang A&F University,Hangzhou 311300,China;Institute of Cotton Research,Chinese Academy of Agricultural Sciences/State Key Laboratory of Cotton Biology,Anyang,Henan 455000,China)
出处
《棉花学报》
CSCD
北大核心
2019年第6期459-473,共15页
Cotton Science
基金
浙江省科技计划项目(2018C26001)
