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类风湿关节炎患者外周血单个核细胞中miR-21表达变化及其意义 被引量:2

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摘要 目的观察类风湿关节炎(RA)患者外周血单个核细胞(PBMC)中miR-21的表达变化,分析其与疾病活动度的相关性。方法RA患者102例,活动期60例(活动期组)、缓解期42例(缓解期组);选择同期健康体检的志愿者50例作为对照组。采用qRT-PCR法检测三组外周血PBMC中的miR-21。采用免疫比浊法检测血清超敏C反应蛋白(hs-CRP)、类风湿因子(RF),ELISA法测定抗环瓜氨酸肽抗体(CCP),魏氏法检测红细胞沉降率(ESR),并进行疾病活动程度评分(DAS28)。采用彩色多普勒超声检查检测双手掌指关节、近端指间关节的滑膜厚度、滑膜血流分级、滑膜动脉RI。结果活动期组外周血PBMC中miR-21相对表达量低于缓解期组,缓解期组低于对照组,两两相比,P均<0.05。活动期组血清hs-CRP、RF、抗CCP、ESR、DAS28评分、滑膜厚度、滑膜血流分级、滑膜动脉RI高于缓解期组(P均<0.05),且缓解期组各指标均高于对照组(P均<0.05)。RA活动期患者外周血PBMC中miR-21与血清hs-CRP、RF、ESR水平及DAS28评分、滑膜厚度呈负相关(r分别为-0.621、-0.639、-0.657、-0.515、-0.559,P均<0.05)。miR-21截断值为0.93时,诊断RA活动期的ROC曲线下面积为0.907,敏感度为91.94%,特异度为88.10%,约登指数为0.800。结论RA患者外周血PBMC中miR-21表达显著降低,且与多种疾病活动度指标呈负相关。miR-21检测有助于RA的诊断及疾病活动度评估。
机构地区 咸阳市中心医院
出处 《山东医药》 CAS 2019年第32期79-81,共3页 Shandong Medical Journal
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