摘要
目的探讨高迁移率族蛋白B1(HMGB1)/Toll样受体4(TLR4)/核因子κB(NF-κB)信号通路在DKD患者中的表达。方法选取2018年1~5月上海市龙华街道社区家庭病床DKD患者80例(DKD组)、单纯T2DM患者80例(T2DM组)及体检健康者80名(NC组)。ELISA法检测HMGB1、TLR4、白介素1β(IL-1β)、IL-6、肿瘤坏死因子α(TNF-α)及hsC-RP;Western blot检测外周血单个核细胞中TLR4和NF-κB的表达。结果 DKD组HMGB1、TLR4、IL-1β、IL-6、TNF-α及hsC-RP高于T2DM组[(96. 69±15. 10)vs(77. 85±14. 73)μg/L,(37. 02±4. 83)vs(30. 95±4. 66)μg/L,(83. 46±28. 19)vs(72. 20±24. 33)ng/L,(39. 66±5. 50)vs(33. 05±4. 81)ng/L,(33. 17±6. 23)vs(31. 09±5. 80)ng/L,(6. 86±1. 13)vs(4. 51±0. 96)mg/L,P<0. 05],外周血单个核细胞中TLR4和NF-κB蛋白表达量升高(P<0. 01)。结论 HMGB1/TLR4/NF-κB信号通路在DKD患者中高表达。
Objective To investigate the expression of HMGB1/TLR4/NF-κB signaling pathway Methods80 DKD patients(DKD group),80 patients with simple T2 DM(T2 DM group)and 80 healthy subjects(NC group)were included in this study. Enzyme linked immunosorbent assay was used to detect the content of serum HMGB1,TLR4,IL-1β,IL-6,TNF-α and hsC-RP. Western blot was used to examine the expression of TLR4 and NF-κB in peripheral ResultsCompared with T2 DM group,the serum levels of HMGB1[(96. 69±15. 10)vs(77. 85±14. 73)μg/L],TLR4[(37. 02±4. 83)vs(30. 95±4. 66)μg/L],IL-1β[(83. 46±28. 19)vs(72. 20±24. 33)ng/L],IL-6[(39. 66±5. 50)vs(33. 05±4. 81)ng/L],TNF-α[(33. 17±6. 23)vs(31. 09±5. 80)ng/L]and hsC-RP[(6. 86±1. 13)vs(4. 51±0. 96)mg/L]increased in DKD patients(P<0. 05),with increased expression of TLR4 and NF-κB in peripheral blood mononuclear cells(P<0. 01).ConclusionThe HMGB1/TLR4/NF-κB signaling pathway is highly expressed in DKD patients.
作者
高希震
陈海冰
蒋丹玛
陈碧华
陈志刚
王青青
GAO Xizhen;CHEN Haibing;JIANG Danma(General Practice of Xuhui District Longhua Community Health Service Center,Shanghai 200232,China)
出处
《中国糖尿病杂志》
CAS
CSCD
北大核心
2019年第11期801-805,共5页
Chinese Journal of Diabetes
基金
上海市卫生和计划生育委员会科研课题（201740268）
