摘要
为了研究诃子(TCR)对低密度脂蛋白(LDL)所含蛋白apoB100氧化修饰的抑制效果,本文采用定性及定量荧光技术对诃子粗提物(CET)及诃子抗氧化有效部位-乙酸乙酯相(EAFT)抑制LDL所含蛋白apoB100氧化修饰的抑制效果进行了研究。结果表明,CET能显著(P<0.05)抑制apoB100中赖氨酸(Lys)游离氨基活性降低,显示出相应的量效关系。在1.25~5.00μg/mL浓度范围内,EAFT对Lys游离氨基活性衰减具有较显著抑制效果(P<0.05);在5~15μg/mL浓度范围内,EAFT对Trp荧光淬灭具有显著抑制作用(P<0.05);在5~15μg/mL浓度范围内,EAFT对总荧光产物及脂褐素的生成也具有显著的抑制作用(P<0.05);三维荧光光谱也直观地表征出EAFT对LDL氧化过程中Peak A所代表的本底物质减少及Peak B所代表的氧化产物的生成具有抑制效果。本文为后续诃子抑制LDL氧化、抗动脉粥样硬化(AS)功能食品研发提供依据。
In order to study the inhibition effect of Terminalia chebula Retz(TCR)on oxidative modification of the protein apoB100 contained in low-density lipoprotein(LDL),this paper studied the inhibition effect of the crude extract of TCR(CET)and the effective anti-oxidation part of TCR(EAFT)on the oxidative modification of the protein apoB100 contained in LDL by using qualitative and quantitative fluorescence techniques.The results showed that,CET could significantly(P<0.05)inhibit the decreasing of free amino activity of Lysine in apoB100,which showed a corresponding dose-effect relationship.The reactivity decreasing of free aminosin Lysine of apoB100 was remarkably inhibited by EAFT within the concentrations of 1.25~5.00μg/mL(P<0.05).Trp fluorescence quenching was markedly inhibited by EAFT within the concentrations of 5~15μg/mL(P<0.05).The generation of the total fluorescence products and lipofuscins were markedly inhibited by EAFT with the same concentrations.The inhibition effect of EAFT was intuitively showed on the three-dimensional fluorescence contour spectroscopy during LDL oxidation.This study would lay the foundation for further research and development of functional foods with antioxidant of LDL and anti-atherosclerosis.
作者
江浩
李佳臻
韩世明
郝澍
张弦
金洪光
江慎华
JIANG Hao;LI Jia-zhen;HAN Shi-ming;HAO Shu;ZHANG Xian;JIN Hong-guang;JIANG Shen-hua(School of Pharmacy and Life Science,Jiujiang University,Jiujiang 332000,China;Department of Pharmacy,Jiangxi University of Traditional Chinese Medicine,Nanchang 330004,China;Jiujiang Andehe Biotechnology Co.,Ltd.,Jiujiang 332000,China;Liupanshui Key Laboratory of Research and Utilization on Characteristic Fruit Tree Resources,School of Biological Science and Technology,Liupanshui Nornal University,Liupanshui 553004,China)
出处
《食品工业科技》
CAS
北大核心
2019年第22期1-6,12,共7页
Science and Technology of Food Industry
基金
国家自然科学基金(31360371,31660104)
江西省重点研发计划(20171BBF60049,20181BBH80010,20192ZDF04032)
江西省教育厅科技项目(GJJ161084,GJJ180918)
江西省卫健委课题(2017B072)
江西省天然产物与功能食品重点实验室开放基金资助项目
六盘水特色果树资源研究与利用重点实验室开放基金(52020-2017-02-03)
九江学院教改课题(2015-04)
