摘要
藻际环境生活着大量微生物,这些微生物对藻类增殖具有重要影响.研究以6种培养基分离源自滇池的1株铜绿微囊藻和2株集胞藻的藻际附着细菌66株,选取其中46株进行16S rRNA基因系统发育分析.它们分属3个门(Actinobacteria、Proteobacteria、Firmicutes),3个纲,4个目,5个科,7个属,9个种. Actinobacteria是优势纲(73.91%),Aeromicrobium是优势属(41.30%),优势种为Aeromicrobium ponti(21.74%). 3株蓝藻藻际附着细菌的优势属均为Aeromicrobium,但Microbacterium和Sphingopyxis只出现在铜绿微囊藻藻际环境中.对46株藻际附着细菌的研究发现,9株对铜绿微囊藻的增殖有显著影响,其中1株促进,8株抑制增殖. L-9-3(Sphingopyxis solisilvae)使铜绿微囊藻的藻细胞数量增加87.62%. NIU-9-2(Sphingopyxis solisilvae)和NIU-M-3(Aeromicrobium halocynthiae)使铜绿微囊藻细胞数量减少81.24%.研究丰富了藻际细菌的资源库,为研究藻-菌相互关系和"以菌控藻"奠定了基础.
A large number of microorganisms live in the phycosphere, which has an important influence on cyanobacteria proliferation. Sixty-six bacteria associated with one strain of Microcystis aeruginosa and two strains of Synechocystis spp. from Dianchi Lake were isolated by using six different mediums. Forty-six bacteria were selected for phylogenetic analysis based on 16 S rRNA gene sequences. The forty-six bacteria belong to three phylums(Actinobacteria, Proteobacteria, Firmicutes), three classes, four orders, six families, seven genus, and nine species. Actinobacteria is the dominant class(73.91%), Aeromicrobium is the dominant genus(41.30%), and the dominant species is Aeromicrobium ponti(21.74%). The dominant genus of the bacteria associated with three strains of cyanobacteria were from Aeromicrobium. However, Microbacterium sp. and Sphingopyxis sp. were only isolated from the phycosphere of Microcystis aeruginosa. Nine strains affected the proliferation of Microcystis aeruginosa significantly, of which one strain showing promotion and eight strains inhibiting proliferation. L-9-3(Sphingopyxis solisilvae) increased the number of cells of Microcystis aeruginosa by 87.62%. NIU-9-2(Sphingopyxis solisilvae) and NIU-M-3(Aeromicrobium halocynthiae) reduced the number of cells by 81.24%.The resource library of bacteria associated with cyanobacteria was enriched, the foundation for studying the interaction between cyanobacteria and their associated bacteria and ‘controlling cyanobacteria by bacteria’ was laid in the study.
作者
李孟珂
夏运生
单壮壮
姬桐
崔晓龙
肖炜
张仕颖
LI Meng-ke;XIA Yun-sheng;SHAN Zhuang-zhuang;JI Tong;CUI Xiao-long;XIAO Wei;ZHANG Shi-ying(School of Resources and Environmental Sciences,Yunnan Agricultural University,Kunming 650201,China;Yunnan Institute of Microbiology,School of Life Sciences,Yunnan University,Kunming 650091,China)
出处
《云南大学学报（自然科学版）》
CAS
CSCD
北大核心
2019年第6期1238-1245,共8页
Journal of Yunnan University(Natural Sciences Edition)
基金
国家自然科学基金(31660042)
关键词
蓝藻
藻际细菌
多样性
铜绿微囊藻
抑藻
cyanobacteria
bacteria associated with cyanobacteria
diversity
Microcystis aeruginosa
cyanobactericidal
