摘要
研究酸浸高压法对未破壁灵芝孢子中灵芝多糖、灵芝总三萜和麦角固醇含量的影响,与未经酸浸高压的未破壁及已破壁的灵芝孢子进行含量比较。以无水葡萄糖为对照品,用紫外–可见分光光度法,对灵芝孢子的灵芝多糖含量进行测定;以齐墩果酸为对照品,用紫外–可见分光光度法,对灵芝孢子的灵芝总三萜含量进行测定;以麦角固醇为对照品,用反相高效液相色谱法,对灵芝孢子的麦角固醇含量进行测定,根据结果对三种成分含量进行比较。结果表明,经酸浸高压的未破壁灵芝孢子,其灵芝多糖的含量明显高于未经酸浸高压的未破壁及已破壁灵芝孢子,其灵芝总三萜和麦角固醇的含量明显高于未经酸浸高压的未破壁灵芝孢子。酸浸高压法可作为一种新的灵芝孢子破壁方法,该法获得的灵芝多糖、灵芝总三萜和麦角固醇的含量明显高于对照组。
The effects of acid leaching high pressure method on the content of ganoderma lucidum polysaccharides,ganoderma lucidum total triterpenoids and ergosterol in unbroken ganoderma lucidum spores were compared with those of unbroken and broken ganoderma lucidum spores without being treated by acid leaching and high pressure.The content of ganoderma lucidum polysaccharides in ganoderma lucidum spores was determined by UV-visible spectrophotometry with anhydrous glucose as reference substance;the content of ganoderma lucidum total triterpenoids in ganoderma lucidum spores was determined by UV-visible spectrophotometry with oleanolic acid as reference substance;the content of ergosterol of Ganoderma lucidum spores was determined by reversed-phase high performance liquid chromatography with ergosterol as a reference substance,and the contents of the three components were compared according to the results.The results showed that for unbroken ganoderma lucidum spores treated by acid-leaching and high-pressure, its content of ganoderma lucidum polysaccharides was significantlyhigher than that of both unbroken and broken ganoderma lucidum spores without being treated by acidleaching and high-pressure, and the content of ganoderma lucidum total triterpenoids and ergosterol wassignificantly higher than that of unbroken ganoderma lucidum spores without being treated by acid leachingand high-pressure. The acid leaching high pressure method can be used as a new method for breaking thewall of ganoderma lucidum spores. The content of ganoderma lucidum polysaccharide, ganoderma lucidumtotal triterpenoids and ergosterol obtained by this method is significantly higher than that of the control group.
作者
吕思敏
何小冰
张丽婷
丁鸿燕
戚怡
吴铁
LV Si-min;HE Xiao-bing;ZHANG Li-ting;DING Hong-yan;QI Yi;WU Tie(Guangdong Medical University Guangdong Runhe Biological Technology Co.,Ltd.Coenzyme Q10 Research Institute,Dongguan Guangdong 523808;Guangdong Medical University,Dongguan Guangdong 523808)
出处
《粮油食品科技》
2019年第6期93-97,共5页
Science and Technology of Cereals,Oils and Foods
基金
广东医科大学·广东润和生物科技有限公司共建辅酶Q10联合研究中心项目(2XX14002)
关键词
酸浸泡
高压
破壁
灵芝孢子
acid leaching
high pressure
wall-breaking
ganoderma lucidum spore
