摘要
目的优选延龄草总皂苷提取纯化工艺,并考察其体内外抗肿瘤活性。方法以出膏率和延龄草总皂苷提取率为指标,单因素、正交实验优化提取工艺;采用大孔吸附树脂技术,通过静、动态吸附与解吸实验,优选最佳纯化工艺;噻唑蓝(MTT)法测定总皂苷对3种肿瘤细胞株MCF-7、HeLa、A549的抑制作用,并研究其对S180小鼠移植瘤的作用。结果最佳提取工艺条件为乙醇体积分数80%,超声时间40 min,料液比1:50,提取温度60℃;纯化工艺为D101大孔树脂,最大上样量为80 mL,洗脱剂为75%乙醇,洗脱体积4 BV,收集洗脱液,纯化得到的固形物中总皂苷含量为61.45%;延龄草总皂苷体外对3种肿瘤细胞均有一定的抑制作用,IC50分别为(7.89±1.13),(8.18±1.04),(28.5±3.16)μg·mL-1,体内对S180荷瘤小鼠的抑制率为12.6%~58.0%。结论该工艺稳定、可行,所得到的总皂苷具有较好的抗肿瘤活性。
Objective To optimize the extraction and purification process of total saponins from Trillium tschonoskii Maxim and to evaluate its antitumor activity in vitro and in vivo. Methods The optimum paste-forming rate and extraction process of total saponins from Trillium tschonoskii Maxim were determined by a single factor and orthogonal experiment.Using macroporous adsorption resin technology, optimized purification process was developed through static and dynamic adsorption and desorption tests.MTT was used to determine the inhibitory effects of total saponins on MCF-7, Hela and A549 cell lines, and the antitumor efficacy of saponins from Trillium tschonoskii Maxim was studied on S180 tumor-bearing mice in vivo. Results The optimum extraction conditions were as follows: ethanol volume concentration 80%, sonication time 40 min, solid to liquid ratio 1:50 and extraction temperature at 60 ℃.The optimized method for purification of saponins was as follows: D101 macroporous absorption resin, maximum sample volume 80 mL, elution with 75% ethanol, the volume 4 BV.Purity of total flavonoids reached 61.45%.The IC50 of antitumor activity of saponins from Trillium tschonoskii Maxim against different tumor cell lines were 7.89±1.13, 8.18±1.04, 28.5±3.16 μg·mL-1, respectively.Tumor growth inhibition ratio of total saponins from Trillium tschonoskii Maxim was between 12.6% and 58.0% in vivo. Conclusion This method was robust and feasible with good separation and purification.The total saponins from Trillium tschonoskii Maxim showed good antitumor effect.
作者
姚敏娜
刘利兵
李晓明
白宏
YAO Minna;LIU Libing;LI Xiaoming;BAI Hong(Medical College of Xi’an Peihua University,Xi’an 710125,China)
出处
《医药导报》
CAS
北大核心
2019年第12期1635-1640,共6页
Herald of Medicine
基金
陕西省教育厅专项科研计划项目(16JK2135)
关键词
延龄草
总皂苷
提取
纯化
抗肿瘤
Trillium tschonoskii Maxim
Total saponins
Extraction
Purification
Antitumor
