摘要
【目的】分析铁皮石斛Dendrobium officinale黄酮类化合物的生物合成途径及相关基因,为铁皮石斛黄酮类化合物代谢调控、药用价值的开发研究提供参考。【方法】利用Illumina HiSeq 4000测序平台对铁皮石斛2个生长阶段茎、叶进行高通量转录组测序,对组装获得的unigenes进行功能注释和黄酮类化合物的生物合成相关基因解析。【结果】铁皮石斛黄酮类化合物代谢相关Unigenes 48个,涉及14个酶。5个CHS相关Unigenes具有CHSlike保守结构域,活性位点氨基酸残基(Cys-His-Asn)高度保守,丙二酰辅酶A结合位点和产物结合位点的部分氨基酸残基发生变异。CHI(Unigene0013781)属于类型I查尔酮异构酶,异构化6′-羟基查尔酮为5-羟基黄烷酮。表达分析表明,2个生长时期的茎和叶,CHS(Unigene0008250)、CHI(Unigene0013781)和F3H的表达量都高于CHS(Unigene0012884)和C3′H。【结论】通过对转录组数据分析,共获得铁皮石斛黄酮类化合物代谢相关Unigenes 48个,涉及14个酶;5个CHS相关Unigenes中,2个编码查尔酮合酶,3个编码联苄合酶;CHI(Unigene0013781)属于类型I查尔酮异构酶。
【Objective】Pathway and genes related to the flavonoid synthesis in Dendrobium officinale were analyzed for studying on the metabolic regulation and medicinal applications of flavonoids.【Method】Transcriptomes of the stems and leaves of D.officinale plants at two growth stages were determined by means of Illumina HiSeq 4000 high-throughput sequencing.The assembled unigenes were used for the functional annotation and genetic analysis of the flavonoid biosynthetic pathway.【Result】There were 48 unigenes involving 14 enzymes found to be associated with the flavonoid metabolism.Among them,the 5 CHS-related unigenes showed a CHS-like conserved domain,highly conserved amino acid residues(Cys-His-Asn)at the active site,and mutated amino acid residues at the malonyl-CoA and product binding sites;and,the CHI(Unigene0013781)was identified as a Type I chalcone isomerase,isomerizing 6'-hydroxychalone to 5-hydroxyflavanone.The expressions of CHS(Unigene0008250),CHI(Unigene0013781)and F3H were higher than those of CHS(Unigene 0012884)and C3′H in the stems and leaves of the plants at both growth stages.【Conclusion】Forty-eight unigenes involving 14 enzymes associated with the flavonoid metabolism in D.officinale plants were identified by the transcriptome analysis.Of the 5 CHS-related unigenes,two encoded chalcone synthase,and 3 bibenzyl synthase,while the CHI(Unigene0013781)a Type I chalcone isomerase.
作者
林江波
王伟英
邹晖
戴艺民
LIN Jiang-bo;WANG Wei-ying;ZOU Hui;DAI Yi-min(Subtropical Agriculture Research Institute,Fujian Academy of Agricultural Sciences,Zhangzhou,Fujian 363005,China)
出处
《福建农业学报》
CAS
CSCD
北大核心
2019年第9期1019-1025,共7页
Fujian Journal of Agricultural Sciences
基金
福建省科技计划公益类专项(2018R1024-3)
关键词
铁皮石斛
转录组
黄酮
功能注释
表达
代谢
Dendrobium officinale
transcriptome
flavonoids
function annotation
expression
metabolism
