摘要
目的:探究白皮杉醇对糖尿病视网膜病变模型大鼠的治疗效果及作用机制。方法:建立糖尿病视网膜病变模型,随机分为模型组、常规用药组、低剂量和高剂量白皮杉醇组各10只。模型组使用100mg/kg的生理盐水灌胃,常规用药组使用100mg/kg的依帕司他灌胃,低剂量、高剂量白皮杉醇组分别使用100、200mg/kg白皮杉醇灌胃。使用光学显微镜观察五组大鼠视网膜组织,Western blot检测五组大鼠视网膜组织中Bax、Bcl-2蛋白的表达,酶联免疫吸附试验法检测五组大鼠视网膜组织VEGF、HIF-1α、ANGⅡ、Ang-1、Ang-2、Tie-2水平。结果:高剂量白皮杉醇组大鼠视网膜组织中Bax、Ang-1/Ang-2比值(1.76±0.05、3.16±0.09)高于低剂量白皮杉醇组(1.01±0.21、2.98±0.02)(P<0.05)。高剂量白皮杉醇组大鼠视网膜组织中Bcl-2、VEGF、HIF-1α、ANGⅡ、Ang-1、Ang-2、Tie-2水平(0.37±0.06ng/mL、121.89±5.45ng/mL、0.38±0.01pg/mL、7.58±0.10ng/mL、8.56±0.04μg/L、3.24±0.25μg/L、3.00±0.04μg/L)低于低剂量白皮杉醇组(0.96±0.21ng/mL、140.25±8.10ng/mL、0.42±0.02pg/mL、8.12±0.09ng/mL、9.10±0.46μg/L、4.12±0.23μg/L、3.46±0.15μg/L)(P<0.05)。结论:白皮杉醇通过作用于Ang/Tie受体信号通路,抑制氧化应激损伤、新生血管的生成,有效保护糖尿病视网膜病变大鼠的视网膜组织,且呈剂量依赖,为临床上治疗糖尿病视网膜病变的治疗提供了理论依据。
AIM:To explore the effect and mechanism of paclitaxel on diabetic retinopathy model rats.METHODS:The diabetic retinopathy model was established and randomly divided into model group,routine drug group,low dose and high dose picetaxel group with 10 rats each.In the model group,100mg/kg normal saline was used for gavage,while in the conventional group,100mg/kg epalrestat was used for gavage.The low dose and high dose picetaxel groups were given 100 and 200mg/kg picetaxel respectively.The retina tissue of five groups of rats was observed by optical microscope,Western blot was used to detect the expression of Bax and Bcl-2 protein,and enzyme-linked immunosorbent assay was used to detect the levels of VEGF,HIF-1α,ANGⅡ,Ang-1,Ang-2 and Tie-2.RESULTS:The ratio of Bax and Ang-1/Ang-2 in the retina of the high dose group was(1.76±0.05,3.16±0.09)higher than that of the low dose group(1.01±0.21,2.98±0.02)(P<0.05).The levels of Bcl-2,VEGF,HIF-1α,ANGⅡ,Ang-1,Ang-2,and Tie-2 in high dose picetaxel group were(0.37±0.06,121.89±5.45ng/mL,0.38±0.01pg/mL,7.58±0.10ng/mL,8.56±0.04μg/L,3.24±0.25μg/L,3.00±0.04μg/L)respectively lower than the lower levels(0.96ng/mL,0.42±0.02pg/mL,8.12±0.09ng/mL,9.10±0.46μg/L,4.12±0.23μg/L,3.46±0.15μg/L)(P<0.05).CONCLUSION:Paclitaxel can inhibit oxidative stress injury and angiogenesis by acting on Ang/Tie receptor signaling pathway,effectively protect retinal tissue of diabetic retinopathy rats in a dose-dependent manner,which provides a theoretical basis for clinical treatment of diabetic retinopathy.
作者
郭丹
杜宁
Dan Guo;Ning Du(Department of Ophthalmology,the Third Affiliated Hospital of Jinzhou Medical University,Jinzhou 121000,Liaoning Province,China;Jinzhou He Eye Hospital,Jinzhou 121000,Liaoning Province,China)
出处
《国际眼科杂志》
CAS
北大核心
2019年第12期2026-2030,共5页
International Eye Science
