摘要
为研究青钱柳(Cyclocarya paliurus)化学成分以及抗氧化活性,以青钱柳70%乙醇提取物为原料,采用正相硅胶柱层析、Sephadex LH-20凝胶柱层析和C18烷基键合硅胶柱层析等方法对青钱柳进行系统的分离和纯化,结合紫外光谱、红外光谱和核磁共振波谱数据,通过与文献比较进行结构鉴定。以Vc为对照,采用1,1-二苯基-2-三硝基苯肼(DPPH)自由基清除法和铁离子还原法(FRAP)比较分离得到的单体化合物的抗氧化活性。分离鉴定了6种化合物分别为香草酸、原儿茶酸、山柰酚、槲皮素、β-谷甾醇和棕榈酸。Vc、原儿茶酸、槲皮素DPPH自由基清除率的IC50值分别为6.58、5.87、5.97μg/m L。原儿茶酸和槲皮素的体外抗氧化能力相近,且都强于Vc。
In order to study the chemical constituents and antioxidant activity of Cyclocarya paliurus. The 70% ethanol extract of Cyclocarya paliurus was used as raw material in this research, the antioxidative fraction of the alcohol extract was subjected to systematic isolation and purification by chromatography techniques such as normal phase silica gel column chromatography, Sephadex LH-20 column chromatography, C18 alkyl bonded silica gel column chromatography. Based on the data obtained by ultraviolet spectrum, infrared spectrum and nuclear magnetic resonance spectroscopy, and identified by comparison with the literature. Vc was used as control, the antioxidant activity of the isolated monomeric comopund was compared by 1,1-diphenyl-2-picrylhydrazyl(DPPH) free radical scavenging method and ferric ion reduction antioxidant power(FRAP) assays. Six compounds were isolated and identified: vanillic acid, protocatechuate acid, kaempferol, quercetin, β-sitosterol and palmitic acid. The IC50 values of DPPH free radical scavenging rates of Vc, protocatechuic acid and quercetin were 6.58 μg/mL, 5.87 μg/mL and 5.97 μg/mL, respectively. The antioxidant activities of protocatechuate acid and quercetin were similar in vitro and stronger than those of Vc.
作者
肖艳华
徐卓
杜治平
XIAO Yanhua;XU Zhuo;DU Zhiping(School of Chemical Engineering&Pharmacy,Wuhan Institute of Technology,Key Laboratory for Green Chemical Process of Ministry of Education,Wuhan 430073)
出处
《食品科技》
CAS
北大核心
2019年第10期223-228,共6页
Food Science and Technology
基金
国家自然科学基金项目(21276201)
湖北省自然科学基金项目(2016CFA079)
关键词
青钱柳
化学成分
抗氧化活性
Cyclocarya paliurus
chemical constituents
antioxidant activity
