摘要
为研究rcsB基因缺失对鸡白痢沙门菌的致病性作用,通过Red同源重组、动物体外及体内试验比较亲本株和缺失株的致病性及生物学特性。结果显示:rcsB基因缺失后,亲本株的生长速度减慢,对肠上皮细胞黏附与侵入能力显著降低,对2日龄SPF鸡的致死性减弱以及在宿主体内生存能力也显著降低。研究调控因子RcsB对致病性的调控机制,可为防控鸡白痢沙门菌病的致病作用等提供了前期数据支撑。
In order to study the pathogenic effect of rcsB gene deletion on Salmonella Pullorum,the pathogenicity and biological characteristics of parent strains and deletion strains were compared by Red homologous recombination and in vitro and in vivo experiments.The results showed that the parent strain was deleted rcsB gene,in which the growth rate was slowed down,the adhesion and invasion ability of intestinal epithelial cells were significantly reduced,the lethality of 2-day-age SPF chickens was reduced,and the viability in the host was also significantly reduced.To study the regulatory factors RcsB to provide a preliminary data support for pathogenic regulation mechanism and prevention and control of the pathogenic role of Pullorum disease.
作者
卢琴
罗青平
张腾飞
罗玲
汪宏才
王红琳
温国元
张蓉蓉
张文婷
邵华斌
LU Qin;LUO Qingping;ZHANG Tengfei;LUO Ling;WANG Hongcai-;WANG Honglin;WEN Guoyuan;ZHANG Rongrong;ZHANG Wenting;SHAO Huabin(Institute of Animal Husbandry and Veterinary,Hubei Academy of Agricultural Scinences,Wuhan,Hubei 430064;Key Laboratory of Prevention and Control Agents for Animal Bacteriosis,Wuhan,Hubei 430064)
出处
《中国家禽》
北大核心
2019年第21期17-21,共5页
China Poultry
基金
国家重点研发计划项目（2016YFD0501305）
现代农业产业技术体系建设专项资金（CARS-42-G13）
农业部重点实验室基金项目（KLPCAAB-YTP-1803）
湖北省农业科技创新中心项目（2016-620-004-003）
湖北省技术创新专项（2018ABA108）
