摘要
以2,3,3-三甲基-3H-苯并[e]吲哚和对二甲氨基苯甲醛为原料,乙醇作溶剂,在酸性催化条件下,通过一步反应合成了比色荧光探针B.在EtOH/HEPES(pH=7. 4)体积比为9∶1的混合体系中,向探针B溶液中加入Cr^3+后,溶液颜色由淡黄色变为紫红色,说明探针B可以对Cr^3+进行裸眼识别.紫外-可见吸收光谱和荧光发射光谱分析表明,探针B对Cr^3+的选择性好、灵敏度高且对EDTA有良好的接力识别.探针B对Cr^3+的结合常数Ka=0.28×10^2mol/L,检出限为1. 90×10^-8mol/L,该检出限低于世界卫生组织(WHO)规定的饮用水中Cr^3+的最大含量(9. 60×10^-8mol/L).利用荧光发射光谱对实际水样中Cr^3+的浓度进行了定量检测.探针B也可应用于对活细胞中Cr^3+的检测,具有较好的应用前景和实用价值.
Colorimetric fluorescence probe B was synthesized from 2,3,3-trimethyl-3 H-benzo[e]indole and p-dimethylaminobenzaldehyde using acidic solvent as catalyst and ethanol as solvent. In the mixed system( p H= 7. 4) of V( EtOH) ∶ V( HEPES) = 9 ∶ 1,the color of solution B changed from pale yellow to purple-red after added Cr^3+ to probe B solution,indicating that probe B can recognize Cr^3+ with naked-eye. Fluorescence emission spectra shows that probe B had good selectivity of Cr^3+ ,high sensitivity and good recognition ability with EDTA. The binding constant( Ka) of probe B on Cr^3+ was 0.28 × 10^2 mol/L and the detection limit was1. 90×10^-8 mol/L. The detection limit was lower than the maximum Cr^3+ content( 9. 60 × 10^-8 mol/L) in drinking water stipulated by WHO. In addition,the concentration of Cr^3+ in actual water samples was quantitatively detected by fluorescence emission spectra. Probe B has been applied to the detection of Cr^3+ in living cells,which has a good application prospect and practical value.
作者
张莹莹
黄译文
赵冰
王丽艳
宋波
ZHANG Yingying;HUANG Yiwen;ZHAO Bing;WANG Liyan;SONG Bo(College of Chemistry and Chemical Engineering,Qiqihar University,Qiqihar 161006,China)
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
2019年第12期2486-2493,共8页
Chemical Journal of Chinese Universities
基金
黑龙江省科学基金留学归国人员科学基金(批准号:LC2017004)
黑龙江省教育厅基本科研业务专项(批准号:135209209)
黑龙江省自然科学基金联合引导项目(批准号:LH2019B021)资助~~
