摘要
目的:探讨microRNA-21、microRNA-25在肺癌组织中的表达及意义。方法:选取肺癌患者85例,采用实时荧光定量PCR方法检测患者癌组织标本(研究组)及其癌旁组织(对照组)中microRNA-21、microRNA-25的表达水平,分析microRNA-21、microRNA-25表达水平及其与临床病理特征的关系,探讨其表达水平与患者预后关系,分析microRNA-21、microRNA-25在肺癌组织中表达的相关性。结果:研究组microRNA-21、microRNA-25 mRNA相对表达水平显著高于对照组,差异有统计学意义(P<0.05);microRNA-21、microRNA-25表达水平与肺癌患者TNM分期有关(P<0.05),与Ⅰ、Ⅱ期患者相比,Ⅲ、Ⅳ期患者癌组织中microRNA-21、microRNA-25表达水平显著上调(P<0.05),而microRNA-21、microRNA-25表达水平与患者性别、年龄、是否吸烟、肿瘤大小、病理类型、淋巴结转移无明显相关性(P>0.05);Kaplan-Meier分析结果显示,肺癌患者癌组织中microRNA-21高表达组中位生存期27.96个月(95%CI:26.79~29.14),5年生存率26.47%,microRNA-21低表达组中位生存期37.42个月(95%CI:33.34~41.51),5年生存率76.47%,microRNA-21高表达组中位生存期显著短于低表达组,差异有统计学意义(Log-rank χ^2=10.53,P<0.05);microRNA-25高表达组中位生存期32.85个月(95%CI:31.28~34.42),5年生存率24.59%,microRNA-25低表达组中位生存期45.22个月(95%CI:42.20~48.24),5年生存率66.67%,microRNA-25高表达组中位生存期显著短于低表达组,差异有统计学意义(Log-rank χ^2=17.31,P<0.05);Spearman相关性分析结果显示,肺癌组织中microRNA-21表达与microRNA-25表达呈显著正相关(r=0.645,P<0.05)。结论:microRNA-21、microRNA-25在肺癌组织中表达显著上调,其表达水平与患者临床分期有关,且肺癌组织中microRNA-21表达与microRNA-25表达呈显著正相关。
Objective:To explore the expression and significance of microRNA-21 and microRNA-25 in lung cancer tissues.Methods:Eighty-five patients with lung cancer were enrolled in this study.Real-time quantitative PCR was used to detect the expression levels of microRNA-21 and microRNA-25 in human cancer tissue specimens(study group) and adjacent tissues(control group).The expression levels of microRNA-21 and microRNA-25 and their relationship with clinicopathological features were analyzed,and the relationship between expression levels and patient prognosis was explored,and the correlation between microRNA-21 expression and microRNA-25 expression in lung cancer tissues was analyzed.Results:The relative expression levels of microRNA-21 and microRNA-25 mRNA in study group were significantly higher than those in control group(P<0.05).The expression levels of microRNA-21 and microRNA-25 were correlated with TNM staging of lung cancer patients(P<0.05).Compared with patients with stage I and II,the expression levels of microRNA-21 and microRNA-25 were significantly up-regulated in cancer tissues of stage III and IV patients(P<0.05),but the expression levels of microRNA-21 and microRNA-25 were not significantly correlated with gender,age,smoking or not,tumor size,pathological types and lymph node metastasis(P>0.05).Kaplan-Meier analysis showed that the median survival time and 5-year survival rate of microRNA-21 high-expression group among lung cancer patients were 27.96 months(95 % CI:26.79~29.14) and 26.47%,and the median survival time and 5-year survival rate of microRNA-21 low-expression group were 37.42 months(95% CI:33.34~41.51) and 76.47%,and the median survival time in microRNA-21 high-expression group was significantly shorter than that in low expression group(χ^2=10.53,P<0.05).The median survival time and 5-year survival rate in microRNA-25 high-expression group were 32.85 months(95% CI:31.28~34.42) and 24.59%,and the median survival time and 5-year survival rate in microRNA-25 low-expression group were 45.22 months(95% CI:42.20~48.24) and 66.67%,and the median survival time in microRNA-25 high-expression group was significantly shorter than that in low-expression group(χ^2=17.31,P<0.05).Spearman correlation analysis results showed microRNA-21 expression in lung cancer tissues was significantly positively correlated with microRNA-25 expression(r=0.645,P<0.05).Conclusion:The expressions of microRNA-21 and microRNA-25 are significantly up-regulated in lung cancer tissues,and their expression levels are related to clinical staging of patients.The expression of microRNA-21 in lung cancer tissues is positively correlated with the expression of microRNA-25.
作者
韩乐
陈文娟
雷光焰
赵征
HAN Le;CHEN Wenjuan;LEI Guangyan(Department of Chest,Shaanxi Cancer Hospital,Xi’an 710061)
出处
《陕西医学杂志》
CAS
2019年第12期1596-1600,共5页
Shaanxi Medical Journal
基金
陕西省卫生健康委员会科研项目(2018D032)
