摘要
叶柄角是大豆株型的重要构成因素,影响大豆冠层结构、光合作用效率以及最终产量。解析大豆叶柄角的遗传基础对提升大豆产量具有重要意义。本研究以2个叶柄角具有显著差异的亲本BLA和SLA以及它们衍生的RIL群体为材料,构建高密度的遗传图谱,对大豆不同部位的叶柄角进行QTL分析,并利用近等基因系验证部分QTL。遗传分析结果显示,叶柄角呈连续正态分布,符合数量性状遗传特征。利用GBS技术构建了包含859个Bin标记的大豆高密度遗传图谱,总遗传长度为2326.9cM,标记间平均距离为2.763cM;共检测到14个调控叶柄角的QTL,LOD值在2.58~4.80之间,可解释遗传变异范围在6.9%~12.4%之间,其中5个QTL定位在第12染色体上且成簇存在;构建的qLA12和qLA18的近等基因系表型结果显示,叶柄角在同一对近等基因家系间差异显著,表明qLA12和qLA18是2个可信的QTL。本研究为进一步克隆调控叶柄角功能基因奠定了基础,为大豆理想株型育种提供了遗传材料。
Leafstalk angle is one of the most important elements for shoot architecture of soybean, affecting canopy architecture, photosynthetic efficiency and final grain yield. Exploring genetic basis of soybean leafstalk angle is significant to improve soybean yield. In this study, two soybean accessions BLA and SLA, contrasting in leafstalk angle, and their derived RIL population were used for high resolution genetic map construction and QTL detection for leafstalk angle, further the near-isogenic lines(NIL) were constructed to validate partial QTLs. Genetic analysis results showed that values of leafstalk angle performed serial and normal distribution which coincides with genetic characteristics of quantitative traits. Additionally, a high resolution genetic map consisting of 859 bin markers was constructed by using GBS method. The linkage map covered 2326.9 cM of genetic distance and the average distance between two markers was 2.763 cM. A total of 14 QTLs for regulating leafstalk angle were detected, with explained 6.9%-12.4% of genetic variation, and their LOD values varied from 2.58 to 4.80, and five of them were clustered together on Chromosome 12. The phenotype of the NILs for qLA12 and q LA18 revealed that leafstalk angle performed significant difference between same pair of NILs which strongly suggested that q LA12 and q LA18 are two believable QTLs. In summary, our results lay a foundation for cloning functional genes of regulating leafstalk angle and provide genetic resources for breeding elite soybean varieties with ideal shoot architecture.
作者
王存虎
刘东
许锐能
杨永庆
廖红
WANG Cun-Hu;LIU Dong;XU Rui-Neng;YANG Yong-Qing;LIAO Hong(College of Resources and Environment,Fujian Agriculture and Forestry University/Root Biology Center,Fuzhou 350002,Fujian,China)
出处
《作物学报》
CAS
CSCD
北大核心
2020年第1期9-19,共11页
Acta Agronomica Sinica
基金
国家自然科学基金项目(31830083)
福建农林大学科技创新专项基金项目(CXZX2018028)资助~~
关键词
大豆株型
叶柄角
QTL定位
近等基因系
soybean shoot architecture
leafstalk angle
QTL mapping
near-isogenic lines(NIL)
