摘要
目的观察推拿对兔腰椎间盘退变组织中TNF-α、IL-6、TGF-β1、CTGF的影响。方法30只白兔随机分为空白组、模型组、推拿组,每组10只。采用经皮穿刺纤维环法将模型组和推拿组建立兔椎间盘退变模型,推拿组进行推拿手法干预。6周后取出椎间盘组织,采用HE染色检测椎间盘组织形态学变化;采用酶联免疫吸附法(ELISA法)检测TNF-α、IL-6、TGF-β1、CTGF的表达水平。结果与空白组比较,模型组和推拿组的椎间盘组织形态学评分、TNF-α、IL-6、TGF-β1、CTGF表达均明显增加(P<0.05);与模型组比较,推拿组TNF-α、IL-6、TGF-β1、CTGF表达均明显降低(P<0.05)。结论推拿可能通过减少TNF-α、IL-6、TGF-β1、CTGF表达而改善椎间盘退变。
Objective To observe the effect of Tuina on the expression of TNF-α,IL-6,TGF-β1 and CTGF in the degenerative tissue of rabbits′lumbar intervertebral disc.Methods A total of 30 white rabbits were randomly divided into the blank group,model group and Tuina group with 10 rabbits in each group.The degeneration models of rabbits′lumbar intervertebral disc were established by percutaneously puncturing the anulus fibrosus in the model group and Tuina group.Then the Tuina group was given the Tuina intervention.The lumbar intervertebral disc tissues of all the rabbits were taken out after 6 weeks.The HE staining method was used to detect the morphological changes of the lumbar intervertebral disc,and the enzyme-linked immunosorbent assay(ELISA)method was used to detect the expression of TNF-α,IL-6,TGF-β1 and CTGF.Results Compared with the blank group,the morphological scores of the lumbar intervertebral discs and the expression levels of TNF-α,IL-6,TGF-β1 and CTGF were significantly increased in the model group and Tuina group(P<0.05).Compared with the model group,the expression levels of TNF-α,IL-6,TGF-β1 and CTGF were significantly decreased in the Tuina group(P<0.05).Conclusion Tuina may improve lumbar intervertebral disc degeneration by reducing the expression of TNF-α,IL-6,TGF-β1 and CTGF.
作者
赵小艳
苏程果
刘华辉
罗才贵
袁晔
朱明双
Zhao Xiaoyan;Su Chengguo;Liu Huahui;Luo Caigui;Yuan Ye;Zhu Mingshuang(Affiliated Hospital of Chengdu University of Traditional Chinese Medicine,Chengdu 610075,China;Sichuan Gem Flower Hospital,Chengdu 610213,China)
出处
《成都医学院学报》
CAS
2019年第6期741-745,共5页
Journal of Chengdu Medical College
基金
四川省中医药管理局课题(No:2018JC016)
成都中医药大学校基金项目(No:ZRYY1711)
关键词
椎间盘退变
推拿
细胞因子
酶联免疫吸附法
Lumbar intervertebral disc degeneration
Tuina
Cytokine
Enzyme-linked immunosorbent assay
