摘要
目的观察兔椎间盘退变组织中TGF-β1与Beclin1、LC3的表达。方法制作新西兰大白兔腰椎间盘退变模型,HE染色检测兔椎间盘退变程度。RT-PCR检测TGF-β1与自噬基因LC3、Beclin1的表达。结果与空白组比较,模型组HE染色显示椎间盘组织形态学评分明显增加(P<0.05);RT-PCR显示TGF-β1与Beclin1、LC3基因表达明显上调(P<0.01)。结论兔腰椎间盘退变组织中TGF-β1与Beclin1、LC3基因表达量增加。
Objective To observe the expression of TGF-β1,Beclin1 and LC3 in the degenerative intervertebral disc tissue of rabbits.Methods The New Zealand white rabbits were used to establish the models of lumbar intervertebral disc degeneration and HE staining was used to detect the degree of the intervertebral disc degeneration.The expression of TGF-β1 and the autophagy genes LC3 and Beclin-1 were detected by RT-PCR.Results The results of HE staining showed that the histomorphological scores of the intervertebral disc in the model group increased significantly when compared with the blank group(P<0.05).The results of RT-PCR showed that the expression of TGF-β1,Beclin1 and LC3 was significantly up-regulated(P<0.01).Conclusion The expression of TGF-β1,Beclin1 and LC3 increases in the degenerative intervertebral disc tissue.
作者
苏程果
赵小艳
刘华辉
卢群文
袁晔
罗才贵
朱明双
Su Chengguo;Zhao Xiaoyan;Liu Huahui;Lu Qunwen;Yuan Ye;Luo Caigui;Zhu Mingshuang(Teaching Hospital of Chengdu University of T.C.M,Chengdu 610075,China;Sichuan Gem Flower Hospital,Chengdu 610213,China)
出处
《成都医学院学报》
CAS
2019年第6期754-757,共4页
Journal of Chengdu Medical College
基金
四川省中医药管理局科研项目(No:2018JC016)
成都中医药大学校基金项目(No:ZRYY1711)
关键词
椎间盘退变
自噬基因
转化生长因子
Intervertebral disc degeneration
Autophagy gene
Transforming growth factor