摘要
目的 探讨壳聚糖支架在骨质疏松性骨缺损修复中的应用价值。方法 18只健康雌性新西兰大白兔随机分为三组,包括空白组、模型组与壳聚糖组,每组6只。空白组不建立骨质疏松性骨缺损模型,模型组建立骨质疏松性骨缺损模型,不放入任何材料处理,壳聚糖组建立骨质疏松性骨缺损模型后给予壳聚糖支架处理。测量术前2 d、术后12周三组腰椎骨密度,术后24周的腰椎组织学评分;术后24周的腰椎最大负荷、抗弯曲强度与载荷/位移等生物力学;术前2 d、术后12周采用生物素双抗体夹心酶联免疫吸附法(ELISA)检测骨形成特异性标志物骨特异性碱性磷酸酶(BSAP)和骨吸收特异性标志物Ⅰ型胶原羧基端末肽(CTX)含量。结果 模型组与壳聚糖组术后12周的腰椎骨密度均显著低于对照组(P <0. 05),也低于术前2 d(P <0. 05),与模型组相比,壳聚糖组骨密度显著增加(P <0. 05);模型组与壳聚糖组术后24周的腰椎组织学评分都显著低于空白组(P <0. 05),壳聚糖组评分高于模型组(P <0. 05);模型组与壳聚糖组术后24周的腰椎最大负荷、抗弯曲强度与载荷/位移等生物力学显著低于空白组,壳聚糖组高于模型组(P <0. 05);与空白组和模型组对比,壳聚糖组术后12周血清中BSAP含量显著上升,与空白组对比,模型组术后12周血清中CTX含量显著下降,而与模型组对比,壳多糖组血清中CTX含量显著上升(P <0. 05)。结论 壳聚糖支架在兔骨质疏松性腰椎骨缺损修复中的应用具有良好的生物相容性与安全性,可促进骨缺损后的骨形成和骨吸收,从而提高修复效果,改善腰椎生物力学功能。
Objective To investigate the application effects of chitosan scaffold in the repair of osteoporotic bone defects.Methods 18 healthy female New Zealand white rabbits were randomly divided into three groups,including blank group,model group and chitosan group,with 6 rabbits in each group.The osteoporotic bone defect model were not established in the blank group,and were established osteoporosis in the model group which were not treated with any material.The chitosan group were used established model of osteoporotic bone defect and then treated with chitosan scaffold.The lumbar spine bone mineral density was measured 2 days before surgery and 12 weeks after surgery.The lumbar spine histology score was 24 weeks after operation.The maximum lumbar spine load,bending strength and load/displacement biomechanics were obtained 24 weeks after surgery.Bone-specific alkaline phosphatase(BSAP)and bone resorption-specific marker type I collagen carboxy terminal peptide(CTX)were detected by biotin double antibody sandwich enzyme-linked immunosorbent assay(ELISA)12 weeks after surgery.Results The bone mineral density of the lumbar vertebrae in the model group and the chitosan group was significantly lower than that in the control group at 12 weeks after operation(P<0.05),and also lower than 2 days before surgery(P<0.05),and compared with the model group,the bone mineral density of the chitosan group was significantly increased(P<0.05).The lumbar spine histological scores in the model group and the chitosan group were significantly lower than those in the blank group(P<0.05),and the chitosan group score was higher than the model group(P<0.05).The biomechanics of lumbar spine maximum load,flexural strength and load/displacement at 24 weeks after operation in the model group and chitosan group were significantly lower than those in the blank group(P<0.05),and the chitosan group was higher than the model group(P<0.05).Compared with the blank group and the model group,the BSAP content in the serum of the chitosan group increased significantly at 12 weeks after operation(P<0.05),and compared with the blank group,the CTX content in the serum of the model group decreased significantly at 12 weeks after operation,while compared with the model group,the serum CTX content in the chitosan group increased significantly(P<0.05).Conclusion The application of chitosan scaffold in the repair of osteoporotic lumbar vertebrae bone defect has good biocompatibility and safety,can improve the repair effect and improve the lumbar spine biomechanical function.
作者
刘超
徐飞
陈向波
付军
LIU Chao;XU Fei;CHEN Xiang-bo(Department of Emergency,3201 Hospital Affiliated Xi'an Jiaotong University,Hanzhong Shaanxi 723000,China;Department of Orthopaedics,The Second Hospital of Yulin City,Yulin Shaanxi 719000,China)
出处
《临床和实验医学杂志》
2019年第24期2618-2621,共4页
Journal of Clinical and Experimental Medicine
基金
陕西省社会发展攻关计划项目(编号:2016SF-136)
关键词
兔
壳聚糖支架
骨质疏松
腰椎骨缺损
生物力学
Rabbits
Chitosan scaffold
Osteoporosis
Lumbar vertebrae defect
Biomechanics
