摘要
本研究旨在探讨由生物陶瓷复合材料引入的外源性血管内皮生长因子(VEGF)对颌骨缺损的影响。将20只新西兰白兔随机分为4组:对照组、假手术组、模型组和支架组。正常白兔作为对照组;颌骨缺损的白兔作为模型组;用装载VEGF的聚醚酮/双相生物陶瓷(PEK-BBC)复合材料处理的颌骨缺损白兔作为支架组;接受手术操作但不含修复材料的颌骨缺损白兔作为假手术组。在术后第30天、第60天和第120天,对各组颌骨进行HE染色以考察骨缺损的修复状态。采用RT-PCR、Western blotting和免疫荧光分析以研究术后VEGF的表达。结果显示,对于所有时间点,对照组和假手术组中发现了完整的骨结构,模型组的骨缺损情况没有改善。然而,在支架组中,在60 d和120 d时观察到支架边缘的骨细胞生长。在60 d和120 d时,模型组中的VEGF水平显著低于对照组(p<0.05),然而,支架组VEGF水平显著高于模型组(p<0.05)。在所有时间点,假手术组的VEGF水平与对照组无显著差异(p>0.05)。总之,由生物陶瓷复合材料引入的外源性VEGF能够逆转VEGF表达在骨缺损中的下调,从而加速骨形成并修复骨缺损。
This study was to investigate the effect of exogenous vascular endothelial growth factor(VEGF)introduced by bio-ceramic composites on jaw defects.20 New Zealand white rabbits were randomly divided into 4 groups:control group,sham group,model group and stent group.Normal white rabbits were used as control group;white rabbits with jaw defects were used as model group;white rabbits treated with PEK-BBC composite material loaded with VEGF were used as stent group;the jaw-bearing white rabbit without repair material was used as a sham group.On the 30th,60th,and 120th days after operation,the jaws of each group were HE stained to examine the repair status of the bone defect.RT-PCR,Western blot and immunofluorescence analysis were used to study the expression of postoperative VEGF.The results showed that for all time points,complete bone structures were found in the control group and the sham group,and the bone defect of the model group was not improved.However,in the stent group,bone cell growth at the edge of the stent was observed at 60 and 120 days.At 60 and 120 days,the level ofVEGFin the model groupwas significantlylower than that in the control group(p<0.05).However,the VEGF level in the stent group was significantly higher than that in the model group(p<0.05).At all time points,VEGF levels in the sham group were not significantly different from the control group(p>0.05).In conclusion,exogenous VEGF introduced by bio-ceramic composites can reverse the down-regulation of VEGF expression in bone defects,thereby accelerating bone formation and repairing bone defects.
作者
金永利
Jin Yongli(Department of Eestern Medicine,Affiliated Hospital of Tongliao University for Nationalities,Tongliao,028000)
出处
《基因组学与应用生物学》
CAS
CSCD
北大核心
2019年第10期4780-4784,共5页
Genomics and Applied Biology
关键词
骨缺损
血管内皮生长因子
聚醚酮
双相生物陶瓷
Bone defect
Vascular endothelial growth factor
Polyether ketone
Biphasic bioceramics
