摘要
通过单因素试验和响应面法优化了肥皂荚皂苷的水浸提工艺,分析了皂苷的表面活性性能,并对皂苷结构进行了初步表征,结果表明:肥皂荚皂苷水浸提的最佳工艺条件为2.0 g肥皂荚果皮粉末,提取温度69℃,提取时间11 h 15 min,液料比10∶1(mL∶g),在此条件下,皂苷得率达26.49%。经过大孔吸附树脂纯化后得到60%乙醇洗脱皂苷和90%乙醇洗脱皂苷,其最低表面张力分别为47.43和35.83 mN/m,临界胶束浓度分别为0.35和0.75 g/L,皂苷中总糖分别为58.46%和51.16%。2种皂苷中单糖均为葡萄糖、木糖、鼠李糖和阿拉伯糖等,但单糖含量略有差异。紫外和红外光谱分析表明:2种肥皂荚皂苷样品的紫外最大吸收波长均为470 nm左右,且均具有皂苷的基本红外特征峰。
The water extraction process of saponins from soap pods was optimized by single factor test and response surface method.The surface activity of saponins was analyzed and the structure of saponins was preliminarily characterized. The optimum extraction conditions were as follows: peel powder of soap pods 2.0 g, temperature 69 ℃, time 11 h 15 min, and the ratio of liquid to solid is 10 ∶1(mL ∶g). Under the above conditions, the yield of saponin is 26.49%. The purified saponins of soap pods were separated by 60% ethanol and 90% ethanol elution. The surface tensions were 47.43 and 35.83 m N/m, respectively. The critical micelle concentrations were 0.35 and 0.75 g/L, respectively. The total sugar content of the hydrolysis was 58.46% and 51.16%, respectively. The monosaccharides in the two kinds of saponins include glucose, xylose, rhamnose and arabinose, but the content of monosaccharide is slightly different. The maximum ultraviolet absorption wavelength of two kinds of saponins was about 470 nm. The infrared spectrum analysis shows that the two kinds of saponins both have the basic characteristic peaks of saponins.
作者
吕喆璇
张威伟
刘丹丹
朱莉伟
蒋建新
LU Zhexuan;ZHANG Weiwei;LIU Dandan;ZHU Liwei;JIANG Jianxin(College of Materials Science and Technology,Beijing Forestry University,Engineering Research Center of Forestry Biomass Materials and Energy,Beijing 100083,China)
出处
《林产化学与工业》
EI
CAS
CSCD
北大核心
2019年第6期102-108,共7页
Chemistry and Industry of Forest Products
基金
国家重点研发计划资助项目(2016YFD0600803)
关键词
肥皂荚皂苷
水浸提
响应面优化
表面张力
结构表征
Gleditsia saponin
water extraction
response surface methodology
surface tension
structural characterization
