摘要
目的鉴定营养治疗后妊娠合并糖尿病患者尿液中的生物标志物。方法采用气相色谱-质谱(GC/MS)代谢组学方法,通过对116例妊娠合并糖尿病孕妇均严格控制饮食和运动1周后,其中58例血糖控制不理想者为实验组,另外58例血糖控制理想者为对照组,对2组孕妇均进行尿液代谢组学分析,识别典型的生物标志物。所有数据均采用SPSS 17.0软件进行检验。结果严格饮食和运动治疗后尿液中α-酮戊二酸、3-羟基丁酸、马尿酸、枸橼酸、脯氨酸、苏氨酸、乳酸、泛酸共8种代谢产物尿液浓度两组间比较差异有统计学意义(P<0.05)。故这8种代谢产物可作为评价妊娠合并糖尿病营养治疗效果的指标。结论α-酮戊二酸、3-羟基丁酸、马尿酸、枸橼酸、脯氨酸、苏氨酸、乳酸、泛酸这8种代谢产物可作为评价妊娠合并糖尿病营养治疗效果的指标。
Objective To identify the bio-markers in the urine of pregnant women with diabetes mellitus after nutritional treatment. Methods The metabonomics method of gas chromatography-mass spectrometry(GC/MS) was used in this study. Strict control of diet and exercise for 1 week in 116 pregnant women with diabetes mellitus Based on 116 cases of gestational diabetes mellitus pregnant women were strictly controlled diet ande xercise for 1 week,58 cases of unsatisfactory blood glucose control as experimental group and 58 cases of satisfactory blood glucose control as control group. Urine metabolomics analysis was performed on both groups of pregnant women to identify typical biomarkers. All data were analyzed by using SPSS 17. 0. Results After strict diet and exercise treatment,the urine concentration of alpha-ketoglutaric acid,3-hydroxybutyric acid,equuric acid,citric acid,proline,threonine,lactic acid and pantothenic acid 8 kinds of metabolites were significant difference between the two groups,(P < 0. 05). Therefore,the8 metabolites can be used as the indicators to evaluate the effect of nutritional treatment for gestational diabetes mellitus. Conclusion Alpha-ketoglutaric acid,3-hydroxybutyric acid,equuric acid,citric acid,proline,threonine,lactic acid and pantothenic acid can be used as the indicators to evaluate the effect of nutritional treatment for gestational diabetes mellitus.
作者
杨桂莲
王益超
吴颖岚
熊喜悦
贾晓宙
梁好
娄明星
YANG Gui Lian;WANG Yi Chao;WU Ying Lan;XIONG Xi Yue;JI A Xiao Zhou;LIANG Hao;LOU Ming Xing(Maternal and Child Health Care Hospital of Hunan Province,Changsha 410008,China)
出处
《中国妇幼卫生杂志》
2019年第6期32-38,共7页
Chinese Journal of Women and Children Health
基金
中国疾病预防控制中心妇幼保健中心合生元母婴营养与健康研究项目资助(2017FYH018)
