摘要
目的观察小胶质细胞在大鼠模型癫痫持续状态(SE)下的激活状态及不同分型的时空表达.方法应用随机数字表法将60只、Wistar大鼠随机分为对照组、SE开始时(NC组)、SE持续30 min(SE30min组)、SE持续1h(SE1h组)、SE持续2h(SE2h组),每组12只.观察各组大鼠行为学变化和海马组织小胶质细胞的激活状态;检测小胶质细胞标记分子白细胞介素(IL)-6、IL-1β及IL-10、IL-4的表达水平.结果SE组随持续时间延长MG细胞反应增加,以SE2h时活化最为明显.SE1h组、SE2h组M2标记分子IL-10、IL-4蛋白及mRNA表达水平显著高于NC组和SE30min组,M1标记分子IL-6、IL-1β蛋白及mRNA表达水平显著低于NC组和SE30min组(P<0.05).结论在匹罗卡品诱导的癫痫大鼠模型中可见小胶质细胞活化,随癫痫发作时间的延长,小胶质细胞的极化状态逐渐转变为具有神经营养作用的M2型细胞,抗炎因子IL-10、IL-4表达水平升高,促炎性因子IL-6、IL-1β表达减少.
Objective To investigate the activation of microglia in state of continuity(SE)of epileptic rat model and the temporal and spatial expression of different types of microglia.Methods 60 healthy male Wistar rats were randomly divided into control group,SE at the beginning(NC group),SE lasting for 30 minutes(SE30min group),SE lasting for 1 hour(SEI h group),SE holding.For 2 hours(SE2h),12 rats in each group.Behavioral changes and activation of microglia in hippocampus were observed.The expression levels of IL-6,IL-1 beta and IL-10 and IL-4 were detected.Results In the SE group,with the prolongation of time,the response of MG cells increased,and the activation of MG cells was most obvious in SE2h group.The expression levels of IL-10,IL-4 prot&n and mRNA in SElh group and SE2h group were significantly higher than those in NC group and SE30min group,while the expression levels of IL-6,IL-1 beta protein and mRNA in Ml group were significantly lower than those in NC group and SE30min group(P<0.05).Conclusion Activation of microglia can be seen in pilocarpine-induced epilepsy rat model.With the prolongation of seizure time,polarization of microglia gradually transforms into neurotrophic M2 cells.The expression levels of anti-inflammatory factors IL-10 and IL-4 will increase,while the expression of pro-inflammatory factors IL-6 and IL-lbeta will decrease.
作者
叶子
马宝君
王蕾
张毅
毛颖
Ye Zi;Ma Baojun;Wang Lei;Zhang Yi;Mao Ying(Departmeni of Neurosurgery,First People's Hospital of Nantong City y 226000,China;Department of Emergency,the First People's Hospital of Nantong City,226000,China;Department of Neurosurgery,Huashan Hospital,Fudan University,Shanghai 200040,China)
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2019年第12期2214-2216,共3页
Chinese Journal of Experimental Surgery
基金
江苏省科技厅自然科学基金(BK20161290)
江苏省“六大人才高峰”高层次人才项目(2019-WSW-199)
南通市科技计划项目(JC2018088 JCZ18004)。
