摘要
水稻(Oryza sativa L.)胚乳的发育状况直接影响着水稻的产量和品质,而胚乳发育过程中复杂的分子机制尚不明确.为揭示水稻胚乳发育过程基因的表达模式,挖掘胚乳发育过程不同时期可能的关键基因,以水稻日本晴开花后(0day after flower,0DAF)籽粒中的子房和开花后第3天、第9天、第16天(3DAF、9DAF、16DAF)籽粒中的胚乳细胞基因芯片表达谱数据为研究对象,筛选并分析了不同时期表达量存在显著差异的基因.结果表明:同处于乳熟期的胚乳细胞(开花后第3天和第9天)基因表达模式最相似,间隔的天数越多,基因表达模式的差异程度越大;开花后第0~3天胚乳细胞消耗大量的能量,且在第3天达到峰值;胚乳中营养物质的积累从开花后第3天开始,第9天时积累最盛,到第16天时物质积累基本完成.加权基因共表达网络分析(WGCNA)发现,不同时期可能的关键基因具有不同的功能,涉及的生物过程包括叶绿素合成、活性氧清除、激素调控和脂质代谢.旨在揭示水稻籽粒开花时到胚乳大体完成增长时胚乳发育相关基因的表达模式,为培育产量高、品质优的水稻品种奠定分子基础.
Rice(Oryza sativa L.)yield and quality is directly affected by the development status of endosperm,while the complex molecular mechanism of endosperm development is still unclear.To reveal the gene expression pattern of the endosperm development process in rice and explore the key genes in different stages of endosperm development,the gene expression microarray data from ovary right after flowering(0 day after flower,0 DAF)and the endosperm on 3 rd,9 th and 16 th day after flowering(3 DAF,9 DAF and 16 DAF)were used in this study,the genes with different expression levels at different stages were then detected and analysed.Results showed that the gene expression pattern of endosperm at the same stage(3 and 9 days after anthesis)were most similar,and the more days the interval was,the greater the difference of gene expression pattern was.The endosperm cells consumed a great deal of energy on the 0 DAF^3 DAF,and the accumulation of nutrients in the endosperm began at 3 DAF,reached its peak on 9 DAF,and basically completed on 16 DAF.Weighted gene co-expression network analysis(WGCNA)manifested that the key genes at different stages had different functions and involved in different biological processes including chlorophyll synthesis,active oxygen equilibrium,hormone regulation and lipid metabolism.The aim of this study was to reveal the expression pattern of genes associated with endosperm development,further to lay a molecular foundation for cultivating rice variaties with high yield and superior quality.
作者
贺超
付沛
王凯
蒋龙
陈慧珍
HE Chao;FU Pei;WANG Kai;JIANG Long;CHEN Huizhen(Jiangxi Agricultural University Key Laboratory of Crop Physiology,Ecology and Genetic Breeding,Ministry of Education,Nanchang 330045,China;Pingxiang Municipal Institute of Agricultural Science,Pingxiang 337000,China)Rice(Oryza sativa L.)
出处
《辽宁师范大学学报（自然科学版）》
CAS
2019年第4期498-512,共15页
Journal of Liaoning Normal University:Natural Science Edition
基金
江西省重大研发专项项目（20165ABC28008）
江西现代农业科研协同创新专项项目（JXXTCX2015001-002-03）
国家水稻产业技术体系项目（CARS-01）
江西省水稻产业技术体系专项项目（JXARS-02-10）
