摘要
建立了超高效液相色谱-串联质谱联用(UPLC-MS/MS)快速测定胶基型嚼烟中7种人工合成甜味剂(安赛蜜、甜蜜素、糖精钠、阿斯巴甜、纽甜、三氯蔗糖和甜菊糖苷)和6种天然甜味剂(赤藓糖醇、木糖醇、甘露糖醇、山梨糖醇、麦芽糖醇和异麦芽酮糖醇)的方法。将胶基型嚼烟溶解在正己烷中,用水提取,再经正己烷快速净化后,人工合成甜味剂在反相柱上以甲醇-水为流动相进行分离,天然甜味剂在专用糖柱上以乙腈-水溶液为流动相进行分离,采用电喷雾离子源以负离子串联质谱多反应监测模式进行检测。结果表明:①13种甜味剂标准曲线的线性关系良好(R2>0.994),检出限在6.8×10-6~0.30μg/kg之间;定量限在2.3×10-5~1.0μg/kg;各甜味剂的线性范围在0.001~1.0μg/mL之间。②高、中、低3个加标浓度样品回收率范围为80.2%~97.6%。③方法的日间精密度和日内精密度均小于10%(n=6)。该方法可用于新型胶基型嚼烟中甜味剂的快速定性定量分析。
To rapidly determine seven synthetic sweeteners(Acesulfame potassium,Sodium cyclamate,Saccharin sodium,Aspartame,Neotame,Sucralose and Stevioside)and six natural sweeteners(Erythritol,Xylitol,Mannitol,Sorbitol,Maltitol and Isomaltitol)in gum-based chewing tobacco,an ultra-high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)method was developed.Samples of gum-based chewing tobacco were extracted by shaking with n-hexane and water.The seven synthetic sweeteners were separated by reversed phase column using gradient methanol/water as mobile phase.The six natural sweeteners were separated by special sugar column using gradient acetonitrile/water solution as mobile phase.All the sweeteners were analyzed by UPLC-MS/MS under the negative ion monitoring mode.The results showed that:1)The linear correlation of the thirteen sweeteners were good(R2>0.994),the limits of detection and quantitation were 6.8×10-6-0.30 and 2.3×10-5-1.0μg/kg,respectively;the linear ranges of all the sweeteners ranged from 0.001 to 1.0μg/mL.2)The recoveries of the sweeteners were from 80.2%to 97.6%.3)The precisions of intra-and inter-day were all less than 10%(n=6).This method is suitable for the rapid qualitative and quantitative analysis of sweeteners in gum-based chewing tobacco.
作者
付强
李国政
林奕云
杨继
蔡群娣
张东豫
邱建华
FU Qiang;LI Guozheng;LIN Yiyun;YANG Ji;CAI Qundi;ZHANG Dongyu;QIU Jianhua(Guangdong Provincial Key Laboratory of Emergency Test for Dangerous Chemicals,Guangdong Institute of Analysis,Guangzhou 510070,China;Technology Center,China Tobacco Henan industrial Co.,Ltd.,Zhengzhou 450000,China;Technology Center,China Tobacco Yunnan Industrial Co.,Ltd.,Kunming 650031,China)
出处
《烟草科技》
EI
CAS
CSCD
北大核心
2019年第12期31-38,共8页
Tobacco Science & Technology
基金
广东省自然科学基金“新型聚合物整体柱的制备与在线固相萃取-液质联用农兽药分析方法学研究”（2015A030310466）
关键词
胶基型嚼烟
超高效液相色谱-串联质谱
合成甜味剂
天然甜味剂
Gum-based chewing tobacco
Ultra-high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)
Synthetic sweetener
Natural sweetener
