摘要
研究花鲈生态冰温无水活运技术,在暂养过程添加VC和运输过程中加入保护液,研究其对花鲈血液生化指标、鳃组织Na^+/K^+-ATPase活力、血清溶菌酶质量浓度、免疫球蛋白M(immunoglobulin M,IgM)水平等鱼体指标的影响。结果表明:花鲈鱼暂养水中加入30 mg/L VC,暂养12 h,冷驯化后包装前浸入G2(0.09%(质量分数,下同)溶菌酶+0.3%山梨糖醇+0.08%姜液)保护液中2-3 s,取出独立包装后进行无水活运的保活效果最好。C2G2保护液(30 mg/L VC(暂养水)结合G2保护液)使用组花鲈在保活运输过程中血清中的总蛋白和白蛋白质量浓度保持稳定,且高于其他保护液处理组,血液中溶菌酶、IgM及鳃中Na+/K+-ATPase也得以保存,使用保护液可有效降低花鲈鳃、肾功能损伤,在花鲈体表形成一层保护膜的同时也有利于鱼体将代谢废物排出。使用保护液可使花鲈无水保活运输后死亡率降低1%-5%。
We investigated the waterless live transportation of Lateolabrax maculatus at ecological ice temperature.The effect of temporary culture with vitamin C(VC)and dipping in protective solutions before subsequent transportation on blood biochemical indexes,gill Na^+/K^+-ATPase(NKA)activity,serum lysozyme concentration and immunoglobulin M(IgM)level of Chinese sea perch was evaluated.The results showed temporary culture with 30 mg/L VC for 12 h followed by cold acclimation and immersion in protective solution G2(0.09%lysozyme+0.3%sorbitol+0.08%Zingiber of ficinale juice)for 23 s before separate packaging was the most effective at keeping fish alive during subsequent transportation.The combination of temporary culture with 30 mg/L VC and protective solution G2 could maintain the serum concentration of total protein and albumin at levels higher than those observed with the other treatments.In addition,serum lysozyme,IgM and gill NKA activity were maintained.The application of the protective solution could effectively attenuate gill and renal damages,form a protective lm on the body surface of sh and facilitate the excretion of metabolic waste,resulting in a 1%5%reduction in the mortality rate after waterless transportation.
作者
张玉晗
谢晶
ZHANG Yuhan;XIE Jing(Shanghai Aquatic Products Processing and Storage Engineering Technology Research Center,Shanghai Professional Technology Service Platform on Cold Chain Equipment Performance and Energy Saving Evaluation,National Experimental Teaching Demonstration Center for Food Science and Engineering(Shanghai Ocean University),College of Food Science and Technology,Shanghai Ocean University,Shanghai 201306,China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2019年第23期262-268,共7页
Food Science
基金
现代农业产业技术体系建设专项（CARS-47)
2019年上海市科技兴农重点攻关项目（2019-02-08-00-10-F01143）
上海市科委平台能力建设项目（19DZ2284000）
上海市科委公共服务平台建设项目（17DZ2293400）
关键词
花鲈
无水活运
暂养
保护液
姜
溶菌酶
山梨糖醇
Lateolabrax maculatus
waterless transportation
temporary culture
protective solution
Zingiber officinale
lysozyme
sorbitol
